山东大学：《医学机能学实验》课程教学课件（PPT讲稿）03 呼吸调节

REGULATIONOFRESPIRATION

REGULATION OF RESPIRATION

outlineTheoryPreparationsbeforeoperationOperationinneckItem

Theory Preparations before operation Operation in neck Item outline

I TheoryThe activity of the respiratory centers regulate the movement ofrespiration. The respiratory centre activity in turn is influenced byafferent impulses from the lungs and various other parts of the bodyFactors whichmodifyrespirationare nervousfactors and chemicalfactors (CO2 and O2 tensions or H+ in blood or cerebrospinal fluid)There are many methods of recordingrespiratory movement: directlyrecording the change in airway pressure and electricity in diaphragmIn this experiment we adopt the methods of recording diaphragmmovement

Ⅰ Theory The activity of the respiratory centers regulate the movement of respiration. The respiratory centre activity in turn is influenced by afferent impulses from the lungs and various other parts of the body. Factors which modify respiration are nervous factors and chemical factors (CO2 and O2 tensions or H+ in blood or cerebrospinal fluid). There are many methods of recording respiratory movement: directly recording the change in airway pressure and electricity in diaphragm. In this experiment we adopt the methods of recording diaphragm movement

I TheoryTo observe the effects of some factors onrespiration and grasp the recordingtechnical of respiration movement

Ⅰ Theory To observe the effects of some factors on respiration and grasp the recording technical of respiration movement

t Production of+AnterialPotArlerial Pco,non-co,acidstBrainextracellularfluidPco,↑Arterial [H]+Brain extracellularfluid[H']LCentralchemoreceptorsPeripheral chemoreceptorstFiningtFiring+Firing of medullaryinspiratoryneuronstFining of neuronsto.diaphragmandinspiratorymtercostalsDiaphragmand Insplratoryintercostals+Contractions+Ventilation

H APPARATUS &AGENTSAPPARATUSa.Mammal animal operating apparatusb.Tracheal cannulac.Syringe(30ml and 50ml respective)d. A rubble tube 50cm in lengthe. A bladder of ball filled with CO2f.Protectiveelectrodeg. Power Lab SystemAGENTS:a.20%urethaneb.0.9% saline solutionc. 3% lactic acid

Ⅱ APPARATUS & AGENTS APPARATUS a. Mammal animal operating apparatus b. Tracheal cannula c. Syringe(30ml and 50ml respective) d. A rubble tube 50cm in length e. A bladder of ball filled with CO2 f. Protective electrode g. Power Lab System AGENTS: a. 20% urethane b. 0.9% saline solution c. 3% lactic acid

III Preparations before operation 1 Anesthesia: 20% urethane (5mg/kg) isadministered through the marginal vein' in theear.The position where we begin to inject anestheticshould be near to distal of marginal ear as possibleas we can.The velocity of injection should be slow and weshould monitor the respiration of the animal

◼ 1 Anesthesia: 20% urethane (5mg/kg) is administered through the marginal vein in the ear. The position where we begin to inject anesthetic should be near to distal of marginal ear as possible as we can. The velocity of injection should be slow and we should monitor the respiration of the animal. Ⅲ Preparations before operation

III Preparations before operation 2 Fixing: After anesthesia, therabbit is fixed.We should lay the rabbit on its back onthe operation table, then fix the limbswith the help of the ropes

Ⅲ Preparations before operation ◼ 2 Fixing: After anesthesia, the rabbit is fixed. We should lay the rabbit on its back on the operation table, then fix the limbs with the help of the ropes

III Preparations before operation 3 Shearing: Before the operationthe hair of the operation site shouldbe sheared.This step we should avoid injuringthe animal's skin

◼ 3 Shearing: Before the operation, the hair of the operation site should be sheared. This step we should avoid injuring the animal’s skin. Ⅲ Preparations before operation

IV Operation in neck 1 Slice the skin: With the scissorsmake a mid incision 3-4 centimeters inlength through the skin over the regionfrom the lower jaw to the breastbone.The anterior chest is now exposed

Ⅳ Operation in neck ◼ 1 Slice the skin: With the scissors make a mid incision 3-4 centimeters in length through the skin over the region from the lower jaw to the breastbone. The anterior chest is now exposed