《植物化学保护》课程教学资源（英文讲稿）Pesticides Analysis with the new Agilent 6220 6460 and 6530

Pesticides Analysis with Screening,Confirmation and the New Agilent 6220 Quantitation LC/MS TOF,6460 Triple Quad and 6530 QTOF CASU Dr.Jerry Zweigenbaum Market Development Specialist April 14,2009 Pesticide Analysis with New Agilent Jet Agilent Technologies Page1

Pesticides Analysis with the New Agilent 6220 Screening, Confirmation and the New Agilent 6220 Quantitation LC/MS TOF, 6460 Triple Quad and 6530 QTOF Quantitation CASU Dr. Jerry Zweigenbaum Market Development Specialist April 14, 2009 Pesticide Analysis with New Agilent Jet Stream Technology Page 1 April 14, 2009

Agilent's 35+Years in Mass Spectrometry Nearly 40,000 systems-Applied to Chromatographic Analysis 5930A 5992A 1971 1976 59388MsD 5971.5975 1988-presen 610 Single Quad 2006,2007 6000 Series LC/MSD Serie 2006-Present 1997-2005 4500,7500 Series ICP/MS 1994-Present ESI-TOF Series lon trap seres 2003-Present 2000-Present 6410 Triple Quad 2006,2007 6510,6520Q-T0F 2006,2007 Pesticide Analysis with New Agilent Jet Page 2 Agilent Technologies a028

Agilent’s 35+ Years in Mass Spectrometry Nearly 40,000 systems – Applied to Chromatographic Analysis 5930A 1971 5992A 1976 5970B MSD 1986 5971 - 5975 1988 - present 6100 Single Quad 2006, 2007 LC/MSD Series 1997-2005 6000 Series 2006-Present 4500, 7500 Series ICP/MS 1994-Present Ion Trap Series 2000-Present ESI-TOF Series 2003-Present 6410 Triple Quad 2006, 2007 6510, Q 6520 Q-TOF 2006, 2007 Page 2 April 14, 2009 Pesticide Analysis with New Agilent Jet Stream Technology

Cutting Edge Triple Quad Q-TOF Performance ASMS 2008 Introductions 1pg-1000:1s/n 6460 Triple Quad With. 6530 Accurate Mass QTOF Agllent Jet Stream Technology Unmatched sensitivity for ultra trace analysis Unique data mining tools for faster compound ID 1pg-50:1s/n Pesticide Analysis with New Agilent Jet AgilentTechnologies Page 3 1.0

Cutting Edge Triple Quad & Q-TOF Performance ASMS 2008 Introductions 6460 Triple Quad 1pg – 1000:1 s/n With. 6530 Accurate Mass QTOF Agilent Jet Stream Technology Unmatched sensitivity for ultra trace analysis 6530 Accurate Mass QTOF trace analysis Unique data mining tools for faster compound ID 1pg – 50:1 s/n 50:1 s/n Page 3 April 14, 2009 Pesticide Analysis with New Agilent Jet Stream Technology

New Agilent 6230 LC/MS TOF Introduced at the Montreux LC/MS Symposium this November Pesticide Analysis with New Agilent Jet Page4 Agilent Technologes m器

New Agilent 6230 LC/MS TOF Introduced at the Introduced at the Montreux Montreux LC/MS Symposium this November Pesticide Analysis with New Agilent Jet Stream Technology Page 4 April 14, 2009

New 6230 TOF and 6530 QTOF Each have a 5 Stage Vacuum System Collision -Free TOF Region 6230 TOF Stage 2.5 x 10-7 Torr 6530 QTOF Stage 1 2 2.5 x 10-7 Torr Pesticide Analysis with New Agilent Jet Page5 菜AgiltTeh

New 6230 TOF and 6530 QTOF Each have a 5 Stage Vacuum System Each have a 5 Stage Vacuum System Collision – Free TOF Region 2 5 10 7 T 6230 TOF Stage 1 2 3 4 5 2.5 x 10-7 Torr 6530 St 1 2 3 4 5 6530 QTOF 2 5 x 10-7 Torr Stage 1 2 3 4 5 2.5 x 10 Torr Page 5 April 14, 2009 Pesticide Analysis with New Agilent Jet Stream Technology

Superheated nitrogen sheath gas Agilent Jet Stream Technology Theory Agilent Technologes Pesticide Analysis with New Agilent Jet Page 6 m器

Agilent Jet Stream Technology Theory Page 6 April 14, 2009 Pesticide Analysis with New Agilent Jet Stream Technology

Agilent Jet Stream:Thermal Gradient Focussing 00 MINUTES Page 7 Agilent Technologies Pesticide Analysis with New Agilent Jet

Agilent Jet Stream: Thermal Gradient Focussing April 14, 2009 Pesticide Analysis with New Agilent Jet Stream Technology Page 7

Agilent Jet Stream lon Generation Thermal Dynamics View This plot is a simulation showing the thermal profile of Agilent Jet Stream Technology LC flow Thermal focusing Super-heated N2 produces the sheath gas most efficient Nebulizer N2 gas desolvation and (near sonic ion generation velocity) possible! 8 650 50 350 Patent Pending Pesticide Analysis with New Agilent Jet Agilent Technologies Page 8 m器

Agilent Jet Stream Ion Generation Thermal D ynamics Vie w This plot is a simulation showing the thermal profile of Agilent Jet Stream Technology LC flow Nebulizer N gas Super-heated N 2 sheath gas Thermal focusing produces the most efficient Nebulizer N2 gas (near sonic velocity) most efficient desolvation and ion generation possibl ! e Patent Pending Page 8 April 14, 2009 Pesticide Analysis with New Agilent Jet Stream Technology

How Does Agilent Jet Stream Work? ●Sample PREVIOUS ●Solvent REPLAYO ●Drying Gas ●Jet Stream Gas Step 8 of 8 Jet Stream Signal Noise 88 Page9 Agilent Technologies Stream Technology April 14.2009

How Does Agilent Jet Stream Work? Page 9 Pesticide Analysis with New Agilent Jet Stream Technology April 14, 2009

SCREENING PESTICIDES WITH LC/TOF Pesticide Analysis with New Agilent Jet Agilent Technologies Page 10 en9W28阁

SCREENING PESTICIDES WITH LC/TOF Pesticide Analysis with New Agilent Jet Stream Technology Page 10 April 14, 2009