复旦大学：《医学英语——学术医学英语口语》学生汇报_Unit 1 Stress and Stress-related Diseases_Stress and serotonin system（1）

David chen 11307120002 STRESS ANR SERQTONIN SYSTEM

STRESS AND SEROTONIN SYSTEM David Chen 11307120002

WHAT IS SEROTONIN x Neurotransmitter(神经递质) x Also called5 hydrotrptamine(5HT)5羟色胺 x Synthesized from tryptophan

WHAT IS SEROTONIN?  Neurotransmitter(神经递质)  Also called 5-hydrotrptamine(5-HT)5-羟色胺  Synthesized from tryptphan

HOW ROES THIS SYSTEM ORK &Q (pre-v 501A 5-HT1B-R post-synaptic pre-synaptic MAOA neuron serotonin neuron degradation 5-HT1A-R ontogeny TPH1 VMAT2 H2-mediated 5-HT2C-R PET-1 i transport SERT synthesis→ 5-HT3A-R MAOA 5-HT1A-R SERT reuptake 5-HT2A-R 5-HT1A-R Fig from Genetic variation in cortico-arhygdala serotonin function and risk for stress- related disease

HOW DOES THIS SYSTEM WORK? Fig. from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease

RISTRIBUTION OF SEROTONERGIC NEURONS IN OUR BRAIN mPFC mPFC内侧前额叶 DRN Amygdala杏仁体 DRN中缝背核 5-HT1A-R 5-HT1B-R amygdala 5-HT2A-R 5-HT2C-R M 5-HT3A-R Fig from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease

DISTRIBUTION OF SEROTONERGIC NEURONS IN OUR BRAIN Fig. from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease mPFC内侧前额叶 Amygdala杏仁体 DRN中缝背核

RELATION BETWEEN SEROTONIN AND STRESS x Exposure to stressors. serotonin neuron activity is increased Excerpted from Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus x...at all levels of the serotonin system.can influence. the response to stress x-Excerpted from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease

RELATION BETWEEN SEROTONIN AND STRESS  Exposure to stressors … serotonin neuron activity is increased…  --Excerpted from Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus  …at all levels of the serotonin system…can influence … the response to stress.  --Excerpted from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease

5-HT1B-R pre-synaptic MAOA neuron 5-HT1A-R TPH1 VMAT2 TPH2-mediated 5-HT2C-R ET- SERT 5-HT3A-R MAOA 5-HT1A-R SERT 5-HT2A-R 5-HT1A-R x Release x Synthesis x Vesicular packaging x Degradation x Receptor

 Release  Synthesis  Vesicular packaging  Degradation  Receptor

5-HT1A-R x Autoreceptor(自受体) on the soma and dendrites of serotonin cells x-EXcerpted from Immunohistochemical evidence of the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei Whose activation inhibits DRN neuronal firing and consequent forebrain release Excerpted from Direct Immunohistochemical evidence of x the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei x Compromised by chronic stress leading to learned helplessness’(习得性无助) x-excerpted from Learned helplessness

5-HT1A-R  Autoreceptor(自受体)on the soma and dendrites of serotonin cells  --Excerpted from Immunohistochemical evidence of the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei  Whose activation inhibits DRN neuronal firing and consequent forebrain release  --Excerpted from Direct Immunohistochemical evidence of  the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei. Compromised by chronic stress leading to ‘learned helplessness’（习得性无助）  --excerpted from Learned helplessness

METHOD x Forced swim test x Evaluation of antidepressants(抗抑郁药)… aimed at rendering or preventing depressive-like states x-Excerpted from The mouse forced swim te x Tail suspension test 150 x Gene knockout luoxetine 0 5 10 (mg/kg)

METHOD  Forced swim test  Evaluation of antidepressants（抗抑郁药）… aimed at rendering or preventing depressive-like states  --Excerpted from The mouse forced swim test.  Tail suspension test  Gene knockout

REFERENCE x [1]Sotelo C, Cholley B, El Mestikawy S, Gozlan H, Hamon M. Direct Immunohistochemical evidence of the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei. Eur Neurosci 1990; 2: 1144 1154 x[2 Seligman ME. Learned helplessness. Annu Rev Med 1972, 23: 407-412 x[3]C Adem et al. The Mouse Forced Swim Test J Vis Exp 2012 Jan 29: (59): e3638 x [4 Andrew Holmes Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease Neurosci Biobehav Rev. 2008 September; 32(7): 1293 1314.doi:10.1016/ i. neubiorev2008.03.006 x[5 Clifford EM, Gartside SE, Umbers V, Cowen PJ, Hajos M, Sharp T Electrophysiological and neurochemical evidence that pindolol has agonist properties at the 5-HT1A autoreceptor in vivo. Br J Pharmacol 1998: 124: 206-212 x[6 Amat J, Baratta M, Paul E, Bland ST, Watkins LR, Maier SF Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus. Nat Neurosci 2005: 8: 365-371

REFERENCE  [1] Sotelo C, Cholley B, El Mestikawy S, Gozlan H, Hamon M. Direct Immunohistochemical evidence of the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei. EurJ Neurosci 1990;2:1144– 1154.  [2] Seligman ME. Learned helplessness. Annu Rev Med 1972;23:407–412  [3] C Adem et al. The Mouse Forced Swim Test J Vis Exp. 2012 Jan 29;(59):e3638  [4] Andrew Holmes Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease Neurosci Biobehav Rev. 2008 September ; 32(7): 1293– 1314. doi:10.1016/j.neubiorev.2008.03.006.  [5] Clifford EM, Gartside SE, Umbers V, Cowen PJ, Hajos M, Sharp T. Electrophysiological and neurochemical evidence that pindolol has agonist properties at the 5-HT1A autoreceptor in vivo. Br J Pharmacol 1998;124:206–212.  [6] Amat J, Baratta MV, Paul E, Bland ST, Watkins LR, Maier SF. Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus. Nat Neurosci 2005;8:365–371

x Any suggestions will be highly appreciated

Any suggestions will be highly appreciated!