《细胞生物学》课程教学资源（PPT课件）第十章 细胞骨架与细胞运动 cell cytoskeleton and cell motility-2/2

Testing and ThinkingImmotile cilia syndrome patients havedefective不动纤毛综合征A.myosin B.tubulin C. dynein D.actinWhat mechanism is responsible formovements of cilia and flagella?A.Kinesin moving on microfilamentsB.Dynein moving on microfilamentsC. Dynein moving on microtubulesD.Myosin moving on microfilaments生1926

1 不动纤毛综合征 Immotile cilia syndrome patients have defective _. A. myosin B. tubulin C. dynein D. actin Testing and Thinking What mechanism is responsible for movements of cilia and flagella? A. Kinesin moving on microfilaments B. Dynein moving on microfilaments C. Dynein moving on microtubules D. Myosin moving on microfilaments

extracellularspaceTesting and ThinkingsecretoryvesiclesplasmaOmembraneOUsethediagramofthe celltoguideyou whenGolgiansweringthefollowingquestions.ConsidertransportvesiclesO(cytosol)the cartoondiagram oftheprimaryaminoacid sequenceofa3o0aminoacidreceptorERnucleusshown below:transmembranecytoplasmicsignalingsignal sequence ligandbinding domaindomaindomain个aminoacid#1aminoacid#300a) label the amino-terminus (N-terminus) and the carboxy-terminus (C-terminus)b)Where would thisproteinbelocatedinthe cell?Where would this protein belocatedif the signal sequence were deleted?What domain is inserted into the ER lumen during synthesis of the堂protein?21926

2 Testing and Thinking Use the diagram of the cell to guide you when answering the following questions. Consider the cartoon diagram of the primary amino acid sequence of a 300 amino acid receptor shown below: a) label the amino-terminus (N-terminus) and the carboxy-terminus (C-terminus) b) Where would this protein be located in the cell? c) Where would this protein be located if the signal sequence were deleted? d) What domain is inserted into the ER lumen during synthesis of the protein?

answertransmembranecytoplasmic signalingCOOHNH2signal sequence ligandbindingdomaindomaindomainK1aminoacid#1aminoacid#300b) Where would this protein be located in the cell?plasma membraneWhere would this protein be located if the signasequence were deleted?cytosold)What domain is inserted into the ER lumenduring synthesis oftheprotein?ligand binding domain堂31926

3 answer a) b) Where would this protein be located in the cell? _ plasma membrane c) Where would this protein be located if the signal sequence were deleted? _ cytosol d) What domain is inserted into the ER lumen during synthesis of the protein?_ ligand binding domain

组成纤毛和鞭毛的轴丝axoneme轴丝的微管结构：9+2·外围：质膜包裹·外周：9组双联管，A管与B管·中央：中央鞘包裹一对微管双联管的结构特点·A管：完全微管、内外动力臂放射辐条·B管：不完全微管?1926

4 组成纤毛和鞭毛的轴丝(axoneme) ◆轴丝的微管结构:9+2 ●外围:质膜包裹 ●外周:9组双联管,A管与B管 ●中央:中央鞘包裹一对微管 ◆双联管的结构特点 ●A管:完全微管、内外动力臂、 放射辐条 ●B管:不完全微管

轴丝微管组成与排列特点·9组三联管：基体（basalbody）.9组双联管：近基体?9+2:轴丝·单管：纤毛顶部BasalbodyCiliumRadialOuterCentralCentraldoubletmicrotubulesheathspokeC&81926

5 ◆轴丝微管组成与排列特点 ●9组三联管:基体(basal body) ●9组双联管:近基体 ●9+2:轴丝 ●单管:纤毛顶部

鞭毛与纤毛鞭毛纤毛3321926

6 鞭毛与纤毛

二联管结构Nexin连丝蛋白1312312动力蛋白内臂4115106动力蛋白外臂一对中央单体微管Spokehead质膜Radial spokeInnersheath放射辐条内鞘5Bridge connectingA管B管central singlets10Doublet microtubule100nm923B二联体微管8411926

7 二联管结构

鞭毛与纤毛如何运动？纤毛动力蛋白（ciliarydynein）多头的动力蛋白基部同A管相连头部同相邻的B管相连头部具有ATP结合位点，水解ATP?1926净

8 ◆纤毛动力蛋白(ciliary dynein) ●多头的动力蛋白 ●基部同A管相连 ●头部同相邻的B管相连 ●头部具有ATP结合位点，水解ATP。 鞭毛与纤毛如何运动？

头与B管连接纤毛动力蛋白南1926

9 纤毛动力蛋白

+纤毛和鞭毛的运动机制微管滑动模型sliding-microtubule model而非微管收缩的结果?101926

10 ◆纤毛和鞭毛的运动机制 微管滑动模型 (sliding-microtubule model) 而非微管收缩的结果