《人体寄生虫学 Human parasitology》课程教学课件（讲稿）Lectures 16 leishmania

Leishmania

Leishmania

IntroductionLeishmaniasisProtozoal disease of mammalsLargely zoonoticVisceral LeishmaniasisMucocutaneous leishmaniasisCutaneous leishmaniasis

Introduction Leishmaniasis Protozoal disease of mammals Largely zoonotic Visceral Leishmaniasis Mucocutaneous leishmaniasis Cutaneous leishmaniasis

Species Pathogenic in HumansLeishmania donovanii (Laveran 1903) (VL)Leishmania tropica (CL)Leishmania major (CL)Leishmania aethiopica (CLLeishmania mexicana (CL)Leishmania brazilliensis (MCL)

Species Pathogenic in Humans Leishmania donovani (Laveran 1903) (VL) Leishmania tropica (CL) Leishmania major (CL) Leishmania aethiopica (CL) Leishmania mexicana (CL) Leishmania brazilliensis (MCL)

eographic distributionofVisceral LeishmaniasisLeishmaniasis is prevalent world wide: ranging fromsouth east Asia, Indo-Pakistan, Mediterranean, north andcentral Africa, and south and central America

Leishmaniasis is prevalent world wide: ranging from south east Asia, Indo-Pakistan, Mediterranean, north and central Africa, and south and central America

MorphologyNonflagellarstageoramastigote（无鞭毛体）Two stagesinlifecycleFlagella stage or promastigote（前鞭毛体）Nonflagellar stage in man and reservoir mammals.Flagella stageinthe sandfly andin cultures.Reproduction occurs by longitudinal binarydivision in both stages

Morphology

Morphology: Promasitgote. Amastigote- Insect- Mammalian stage- Motile- Non-motile- Midgut-Intracellular

Morphology • Promasitgote – Insect – Motile – Midgut • Amastigote – Mammalian stage – Non-motile – Intracellular

MorphologyThe individual amastigoteanteriormeasure approximately 3 μm inFlagellumdiameter. Leishman-Donvanbody.+abThe promastigotes arek1approximately 25 μum in lengthna. Rhizoplastkinetoplastb. Basal bodyk. Kinetoplastamastigotepromastigoten. nucleusposterior

Morphology • The individual amastigote measure approximately 3 µm in diameter. Leishman-Donvan body. • The promastigotes are approximately 25 µm in length. a. Rhizoplast b. Basal body k. Kinetoplast n. nucleus

Promastigote

Promastigote

Electron photomicrograph ofpromastigotes

Electron photomicrograph of promastigotes

EP promastigote rosette1806

EP promastigote rosette