《生物化学》课程PPT教学课件（英文版）chapter 20 carbohydrate biosynthesis

s:Chapter 20 Carbohydrate Biosynthesis 1. Gluconeogenesis: The universal pathway for synthesis of glucose 2. Biosynthesis of glycogen, starch, and sucrose 3. CO2 fixation in plants(the Calvin Cycle) 4. Regulation of carbohydrate metabolism in plants

Chapter 20 Carbohydrate Biosynthesis 1. Gluconeogenesis: The universal pathway for synthesis of glucose. 2. Biosynthesis of glycogen, starch, and sucrose. 3. CO2 fixation in plants (the Calvin Cycle). 4. Regulation of carbohydrate metabolism in plants

1, Carbohydrates are synthesized from simple precursors via gluconeogenesis A few three-carbon compounds(including lactate, pyruvate, glycerol, and 3-phosphoglycerate) serve as the major precursors for carbohydrate (glucose) biosynthesis or gluconeogenesis The reactions of gluconeogenesis are essentially the same in different organisms The conversion of pyruvate to glucose is the central pathway in gluconeogenesis

1. Carbohydrates are synthesized from simple precursors via gluconeogenesis • A few three-carbon compounds (including lactate, pyruvate, glycerol, and 3-phosphoglycerate) serve as the major precursors for carbohydrate (glucose) biosynthesis, or gluconeogenesis. • The reactions of gluconeogenesis are essentially the same in different organisms. • The conversion of pyruvate to glucose is the central pathway in gluconeogenesis

Blood Other glucose Glycoproteins monosaccharides Sucrose Glycogen Disaccharides Glucose 6-phosphate Animals erg Plants Phosphoenolpyruvate Citric acid cycle Pyruvate Glucogenic Glycerol 3-Phosphoglycerate amino Lactate Triacylglycerols ce fixation

2, The opposing pathways of glycolysis and gluconeogenesis have 3 reactions different and 7 reactions in common The reversible reactions between pyruvate and glucose are shared by gluconeogenesis and glycolysis, but the irreversible reactions are different (bypassed? in gluconeogenesis

2. The opposing pathways of glycolysis and gluconeogenesis have 3 reactions different and 7 reactions in common • The reversible reactions between pyruvate and glucose are shared by gluconeogenesis and glycolysis, but the irreversible reactions are different (“bypassed” in gluconeogenesis)

Glyco y UDP Glucose 1-phosphate ATP Third 1, 3-Bisphosphoglycerat ADP ADP ADP Glucose 6-phosphate ATP Glycolysis 3-Phosphoglycerate pathway Glu Fructose 6-phosphate P fructose 2.6- 2-Phosphoglycerate Second AMP,“一→ fructose 2.6- bisphosphate Fructose 1, 6-bisphosphate Glyceraldehyde Dihydroxyacetone GDP 3-phosphate GTP ADP NAD. NADH NADH NADH 1. 3-Bisphosphoglycerate MalateNAD' Opposing pathways of NADt NADH glycolysis and gluconeogenesis Oxaloacetate ADP with 3 different and 7 common ATP Alanine >Pyruvate reactions Pyruvate Alanine

Opposing pathways of glycolysis and gluconeogenesis: with 3 different and 7 common reactions

s3. Pyruvate is converted to phosphoenolpyruvate(PEP)via two alternative paths In both paths, pyr uvate is converted to oxaloacetate with the catalysis of pyruvate carboxylase)in mitochondria In one path, oxaloacetate is converted directly to PEP in the matrix of mitochondria in a reaction catalyzed by the mitochondrial PEP carboxykinase isozyme, PEP is then transported to the cytosol for further conversion

3. Pyruvate is converted to phosphoenoylpyruvate (PEP) via two alternative paths • In both paths, pyruvate is converted to oxaloacetate (with the catalysis of pyruvate carboxylase) in mitochondria. • In one path, oxaloacetate is converted directly to PEP in the matrix of mitochondria in a reaction catalyzed by the mitochondrial PEP carboxykinase isozyme, PEP is then transported to the cytosol for further conversion

In another path, oxaloacetate is first converted to malate in the matrix, which is, then transported to the cytosol. where it is converted to oxaloacetate and then PEP in a reaction catal yzed by cytosolic PEP carboxykinase isozyme Both paths involve a carboxylation decarboxylation sequence, acting as a unique way to activate pyruvate Two high-energy phosphate equivalents must be expended to convert one pyruvate to one PEP

• In another path, oxaloacetate is first converted to malate in the matrix, which is then transported to the cytosol, where it is converted to oxaloacetate, and then PEP in a reaction catalyzed by cytosolic PEP carboxykiase isozyme. • Both paths involve a carboxylation￾decarboxylation sequence, acting as a unique way to activate pyruvate. • Two high-energy phosphate equivalents must be expended to convert one pyruvate to one PEP

PEP From pyruvate Bicarbonate Pyruvate cytosolic PEP to PEP: two HO-C+ CH3-C Oxaloacetate alternative paths ATP NADH+H+ pyruvate cytosolic arboxylnse biotin malate ADP P NAD+ Malate Oxaloacetate Malate PEP NAD+ Oxaloacetate mitochondrial PEP CC-CH2*C-C NADH+H+ carboxykinase Oxaloacetate Oxaloacetate pyruvate carboxylase pyruvate CO rboxylase cO Guanosine 0-P-0-P-0-P-O Pyruvate TP Mitochondrion Cytosol →GDP PEP Pyruvate carboxykinase NADH +H+ 03 lactate dehydrogenase CH,=C-Coo NAD+ Phosphoenolpyruvate Lactate b)

From pyruvate to PEP: two alternative paths

24. Conversion of fructose 1, 6 bisphosphate to fructose 6-phosphate is the second bypassing step The reaction is catalyzed by Mg 2+ -dependent fructose 1, 6-bisphosphatase (instead of phosphofructokinase Fructose-1, 6-bisphosphate H20- fructose-6-phosphate Pi △G° 16. 3 kJ md

4. Conversion of fructose 1,6- bisphosphate to fructose 6-phosphate is the second bypassing step • The reaction is catalyzed by Mg 2+ -dependent fructose 1,6-bisphosphatase (instead of phosphofructokiase-1)

5. The conversion of glucose 6-phosphate to glucose is the last bypassing step The reaction is catalyzed by glucose 6-phosphatase instead of hexokinase). The enzyme is present on the lumen side of the ER membrane of hepatocytes and renal cells The enzyme is not present in muscle or brain cells, where gluconeogenesis does not occur coe6 phosphate+H2O—→ glucose+P1△G=-13.8 kJ/mol

5. The conversion of glucose 6-phosphate to glucose is the last bypassing step • The reaction is catalyzed by glucose 6-phosphatase (instead of hexokiase). • The enzyme is present on the lumen side of the ER membrane of hepatocytes and renal cells. • The enzyme is not present in muscle or brain cells,where gluconeogenesis does not occur