石河子大学：《实验诊断学》课程教学资源（PPT课件讲稿）Unit2 Basic Hematology

附院检验科 Clinical laboratory offirs affiliated hospital Clinical Laboratory Testing Basic Hematology

Clinical Laboratory Testing Basic Hematology

附院检验科 Clinical laboratory offirst affiliated hospita Clinical Laboratory Testing-Basic Hematology Department of Hematology Study of formed elements of blood and blood forming tissues Screen,diagnosis and monitor hematologic diseases (anemias,leukemias,etc.)and also give a general idea of overall health

Clinical Laboratory Testing – Basic Hematology • Department of Hematology – Study of formed elements of blood and blood forming tissues – Screen, diagnosis and monitor hematologic diseases (anemias, leukemias, etc.) and also give a general idea of overall health

附院检验科 Clinical laborato yof first affiliated hospital Clinical Laboratory Testing -Basic Hematology Composition of Blood-blood is 6-8%of total body weight and equals approximately 5 liters (think about 5 liter bottles of Coke)

Clinical Laboratory Testing – Basic Hematology • Composition of Blood - blood is 6-8% of total body weight and equals approximately 5 liters (think about 5 liter bottles of Coke)

附院检验科 Clinical laboratory offirst affiliated hos bod Plasma (percentoge by weight) Proteins 7% Blood Globulins 38 Composition Other fluids and fissues 92% 袋 lons Nutrients Oher solutes 2% Waste podu Gases Formed elements (number per cubicmm) Rogulatory substances. 250-400 fousond Leukocytes Leukocytes Neutrophils 60-70% 2 24% Image 163 Components of blood Copyright2001 by Mosby.Ine

Blood Composition

附院检验科 Clinical laboratoryoffirst affiliated hospital Serum vs Plasma- Both are fluid portions of blood Serum is from clotted blood,has no clotting factors (used up in the clotting process) Plasma is from anticoagulated blood,has clotting factors,makes up about 45-60%of blood's volume

– Serum vs Plasma – • Both are fluid portions of blood • Serum is from clotted blood, has no clotting factors (used up in the clotting process) • Plasma is from anticoagulated blood, has clotting factors, makes up about 45-60% of blood’s volume

一附院检验科 Clinicallaboratoryoffrsffiliated hospital Common Hematology Laboratory Tests Complete Blood Count(CBC)includes: ·RBC count ·VBC count ·Hemoglobin ·Hematocrit ·WBC Differential ·Platelet count Additional information on size and appearance of blood cells

• Common Hematology Laboratory Tests – Complete Blood Count (CBC) includes: • RBC count • WBC count • Hemoglobin • Hematocrit • WBC Differential • Platelet count • Additional information on size and appearance of blood cells

附院检验科 Clinical laboratoryoffrs affiiated hospital Common Hematology Laboratory Tests Erythrocyte Sedimentation Rate (ESR) Reticulocyte Count Sickle Cell Testing Coagulation Tests include: Prothrombin Time (PT) Partial Thromboplastin Time (PTT) ·Fibrinogen 。Factor Analysis

• Common Hematology Laboratory Tests – Erythrocyte Sedimentation Rate (ESR) – Reticulocyte Count – Sickle Cell Testing – Coagulation Tests include: • Prothrombin Time (PT) • Partial Thromboplastin Time (PTT) • Fibrinogen • Factor Analysis

一附院检验科 Clinicallaboratoryoffrsffiliated hospital .Hematological Diseases (Diseases may be classified in more than one category) Diseases of improper or insufficient production Anemias -Examples include iron deficiency anemia and aplastic anemia,among others Leukemias-WBC production is affected and defective. Examples include acute and chronic leukemias Thrombocytopenia -Decreased platelets.Patient may have bleeding problems

• Hematological Diseases (Diseases may be classified in more than one category) – Diseases of improper or insufficient production • Anemias – Examples include iron deficiency anemia and aplastic anemia, among others • Leukemias – WBC production is affected and defective. Examples include acute and chronic leukemias • Thrombocytopenia – Decreased platelets. Patient may have bleeding problems

附院检验科 Clinical laboratoryoffrs affiiated hospital Diseases of defective cell function May be combination of improper cell production and defective function Examples include iron deficiency anemia and certain leukemias Inherited hematological diseases Examples include hemophilia,sickle cell anemia,G6PD deficiency,thalassemias Secondary or acquired hematological diseases Examples include hemolytic disease due to renal pathologies or atypical lymphocytes due to viral infections

– Diseases of defective cell function • May be combination of improper cell production and defective function • Examples include iron deficiency anemia and certain leukemias – Inherited hematological diseases • Examples include hemophilia, sickle cell anemia, G6PD deficiency, thalassemias – Secondary or acquired hematological diseases • Examples include hemolytic disease due to renal pathologies or atypical lymphocytes due to viral infections

一附院检验科 Clinicallaboratory offrst affiliated hospital Cellular Elements-make up the remainder of the blood volume ·Erythrocytes/RBCs -~4-6 million/μL Transport oxygen and carbon dioxide Electron Micrograph Stained Blood Smear Viewed by Microscope

– Cellular Elements – make up the remainder of the blood volume • Erythrocytes / RBCs – ~ 4-6 million/µL – Transport oxygen and carbon dioxide Electron Micrograph Stained Blood Smear Viewed by Microscope