麻省理工大学：《蛋白质标志—分解机制与人类疾病》（英文版）Introduction Session 7

Introduction Session 7 C-O H (E3) Prot DUB Ubl-protein ULP conjugate Reversible ligation of Ubiquitin and Ubls to other proteins. After Figure 1 in Hochstrasser M. Evolution and function of ubiquitin-like protein-conjugation systems Nat Cell Biol. 2000 Aug: 2(8) E153-7 UBI ncnn Mono Endocytosis/lysosomal Histones ion channels Poly (Lvs29) 26S proteasomal degradation? UFD substrates Poly (Lys48) 26S proteasomal degradation Many shon lived proteins Poly (Lys3) Post-replicalive DNA repair. PCNA plasma membrane proleins ISG15(UCRP Scrpin 2a, Statl,ERKl,others AUT7(APGN) GATEI6(Go Phosphalidyleth gABAr light chin 3) ated APG12 oophagy,cytoplasm-to-vacuoleAPG5 NEDDS (RUB 1) Cullins rt, chromosome Mans HUB I Cell Sphl, Hbll Apoptosis Unknown Growth at high temperature Unknown Related Modifier 1) T-cell activation rCRa-like protein Fgiure by MIT OCW. fter Table 1 in Schwartz and Hochstrasser, TIBS 28(2003)321-328

Introduction Session 7 Fgiure by MIT OCW. After Table 1 in Schwartz and Hochstrasser, TIBS 28 (2003) 321-328. Figure by MIT OCW. After Figure 1 in Hochstrasser M. Evolution and function of ubiquitin-like protein-conjugation systems. Nat Cell Biol. 2000 Aug; 2 (8): E153-7

Image removed due to copyright considerations See Figure 2 in Hochstrasser M. Evolution and function of ubiquitin-lik protein-conjugation systems Nat Cell Biol. 2000 Aug; 2(8): E153-7 MocK El El like Fsc like RHD FS-S RHD El- RHD FS RHD HS El- RHD HS. RHDHS RHD上S Potential Parallels between Sulphurtransferases and UBI Transferases After Figure 4 in Hochstrasser M. Evolution and function of ubiquitin-Iil protein-conjugation systems. Nat Cell Biol. 2000 Aug 2( 8): E153-

Image removed due to copyright considerations. See Figure 2 in Hochstrasser M. Evolution and function of ubiquitin-like protein-conjugation systems. Nat Cell Biol. 2000 Aug; 2 (8): E153-7. Figure by MIT OCW. After Figure 4 in Hochstrasser M. Evolution and function of ubiquitin-like protein-conjugation systems. Nat Cell Biol. 2000 Aug ;2 (8): E153-7

E1 BA2 Figure 1 Pathway of SUMO conjugation deconju SUMO head). The conjugation involves SUMO activati enzvme (AOSI/UBA2)and an E2 conjugating enzyme(Ubc9) that form thioesters (S) with the modifier. E3-like factors stimulate the attachment to specific lysine residues within a target protein. The cleavage of SUMO from its target proteins is catalysed by the USP SENP isopeptidase family Courtesy of S. Muller, A Ledl and D Schmidt. Used with permission Source Figures 1 Figure 2, Muller S, Led A, Schmidt D. Related Articles, Links SUMO a regulator of gene expression and genome integrity Oncogene.2004Mar15;23(11):19982008 0B Proteasome Courtesy of The Elso Gazette. Used with permission. Source: Figure 2 in Andreas Gast, Andrea Pichler, Sowmya Swaminathan and Frauke Melchior. SUMO shifts to new functions. In The ELSO Gazette e-magazine of the EuropeanLifeScientistOrganization(http.://ww.the-elso-gazette.org/magazines/issue1/mreviews/mreviews6.asp Issue 1(1 September, 2000) SUMO antagonizing Ubiquitin and substrate phosphorylation favoring ubiquitination over sumoylation. Co Repressor Ubiquitin Gene Activation Figure 2 A he interplay of the SUMO and ubiqui system in trat regulation. PIAS-triggered SUMOyla ecruitment of a transcriptional corepressor leading to gene lencing. Ubiquitination of a transcription factor and or binding f a ubiquitin E3ligase within a transactivation domain can facilitate binding of a coactivator leading to gene activation Courtesy of s. Muller, A Ledl and D Schmidt. Used with ource Figures 1 Figure 2, Muller S, LedI A, Schmidt D. Related Articles, Links SUMO: a regulator of gene expression and genome integrity. Oncogene. 2004 Mar 15: 23(11):19982008

SUMO antagonizing Ubiquitin and substrate phosphorylation favoring ubiquitination over sumoylation. Courtesy of The Elso Gazette. Used with permission. Source: Figure 2 in Andreas Gast, Andrea Pichler, Sowmya Swaminathan and Frauke Melchior. SUMO shifts to new functions. In The ELSO Gazette: e-magazine of the European Life Scientist Organization (http://www.the-elso-gazette.org/magazines/issue1/mreviews/mreviews6.asp), Issue 1 (1 September, 2000) Courtesy of S. Muller, A. Ledl and D. Schmidt. Used with permission. Source Figures 1 / Figure 2, Muller S, Ledl A, Schmidt D. Related Articles, Links SUMO: a regulator of gene expression and genome integrity. Oncogene. 2004 Mar 15; 23 (11): 1998-2008. Courtesy of S. Muller, A. Ledl and D. Schmidt. Used with permission. Source Figures 1 / Figure 2, Muller S, Ledl A, Schmidt D. Related Articles, Links SUMO: a regulator of gene expression and genome integrity.Oncogene. 2004 Mar 15; 23 (11): 1998-2008