华东师范大学：《植物生理学》课程资源（实验课教学课件）植物组织培养——培养基配制 Plant Physiology Experiments Tissue culture——Media preparation

Tissue culture Media preparation Plant Physiology Experiments

Plant Physiology Experiments Tissue culture —— Media preparation

Tissue Culture a technique which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels

Tissue Culture • A technique which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. • By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels

Totipotency An ability to dedifferentiate demonstrates that differentiated plant cells retain all the genetic information required for the development of a complete plant, a property

Totipotency • An ability to dedifferentiate demonstrates that differentiated plant cells retain all the genetic information required for the development of a complete plant, a property

The background I Its origins at the beginning of the 20th century with the work of Gottleib Haberlandt(left plants)and Alexis Carrel(right animals

The Background Ⅰ • Its origins at the beginning of the 20th century with the work of Gottleib Haberlandt (left, plants) and Alexis Carrel (right, animals)

The BackgroundⅡ Folke Karl Skoog su(崔澂) Both organ formation and subsequent development are brought about by quantitative changes in amounts and interactions between nutrients and growth factors which are essential for growth of all cells, so that the pattern of development is determined by the relative supplies.. of these materials at particular loci http://www.nap.edu/readingroom.php?book=biomems&page=fskoog.html

The Background Ⅱ Folke Karl Skoog Tsui(崔澂) Both organ formation and subsequent development are brought about by quantitative changes in amounts and interactions between nutrients and growth factors which are essential for growth of all cells, so that the pattern of development is determined by the relative supplies . . . of these materials at particular loci http://www.nap.edu/readingroom.php?book=biomems&page=fskoog.html

The backaround亚 Experimental results on the establishment of the N6 medium are described on its application to anther culture of cereal crops is reviewed The induction frequency of pollen plants in rice, Wheat, triticale, rye and maize was higher on N6 than on miller' s or ms medⅰa ChuC-C(朱至清)

The Background Ⅲ • Experimental results on the establishment of the N6 medium are described on its application to anther culture of cereal crops is reviewed. • The induction frequency of pollen plants in rice, wheat, triticale, rye and maize was higher on N6 than on Miller's or MS media. Chu C-C （朱至清）

Tobacco (A、B、C、 D for different growth periods)

A B C D Tobacco （A、B、C、D for different growth periods）

1

Rice genetic transfomation Tion Ion

callus induction callus co-culture the first selection rootage redifferentiation the second selection Rice genetic transfomation

Why do plant Tissue Culture? Fast propagation and virus remova 2. Anther and Pollen culture 3. Somaclonal variation and mutant separation 4. Protoplast culture and cell hybridation Chinesecropgermplasmresourcesinformationsystemhttp://icgr.caas.net.cn/ Plant in vitro germplasm bank http://www.ib.cas.cn/jgsz/zhicheng/sypt/200906/t20090603276331.html produce dary metabolite 7. Plant tissue banks *, can be frozen then regenerated through tissue culture

Why do Plant Tissue Culture? 1. Fast propagation and virus removal. 2. Anther and Pollen culture. 3. Somaclonal variation and mutant separation. 4. Protoplast culture and cell hybridation. 5. Plant artificial seed, or man-made seed. 6. Give a continuous supply of young plants to produce secondary metabolite. 7. Plant ‘tissue banks*’ can be frozen, then regenerated through tissue culture. Chinese crop germplasm resources information system: http://icgr.caas.net.cn/ Plant in vitro germplasm bank : http://www.ib.cas.cn/jgsz/zhicheng/sypt/200906/t20090603_276331.html