麻省理工大学：《生物工程》教学讲义（英文版）lec 6 slides

VORM LIKE 土 RIN LAS[l< F ILAMENTS s √s Bending Emrst= 2 E工 dNs.F v ERSIS TENCE Le心T计 <t(s).t(s+as))=exp △s E工 P BENDIN E NERo 2 E t见 &s 2 RELATION。= WLc E FsC <&7=2 2 P exP LC P P <R27→L ti ble KR2722PL P

dsDNa fit to WLc model Hooke’slaw 0.01 0.4 0.8 Extension(X/L) After Bustamante et al., Current Opinion in Structural Biology, 2001

FJC dsDNA 100 10 1 0.1 0.01 Hooke’s law fit to WLC model Force (pN) 0 0.2 0.4 0.6 0.8 1.0 1.2 Extension (x/L) After Bustamante et al., Current Opinion in Structural Biology, 2001

8760 Marko and Siggia (Mac rowekecules (qas) Graph( Figure 2. )removed due to copyright considerations Tep/t( 2 ds D: fficul. in Me ra Marke 4 siggi (ins): limit> for P/L L

Graph (Figure 2.) removed due to copyright considerations

Images removed due to copyright considerations See Figures 1, 2, and 3 and Table 1 in Isambert, Herve, et al Flexibility of Actin Filaments Derived from Thermal Fluctuations Journal of Biological Chemistry 270(19)12 May 1995, 11437-11444

Images removed due to copyright considerations. See Figures 1, 2, and 3 and Table 1 in Isambert, Herve, et al. "Flexibility of Actin Filaments Derived from Thermal Fluctuations." Journal of Biological Chemistry 270 (19) 12 May 1995, 11437-11444

After Fig. 1 and 2 in Sun, Y et al. "Mechanical Properties of Single Type ll Collagen Molecule Paper No: 0082, 48th Annual Meeting of the Orthopaedic Research Society Stretching a procollagen ll molecule with an optical tweezers Cover glass Procollagen Cover glass XY stage Laser light The force-extension curve of a single collagen II 50100150200150300350 Extensions(nm) Image removed due to copyright considerations Figure 1 in Fuji, T et al. "Mechanical Properties of Single Hyaluronan Molecules. Paper No: 0390, 48th Annual Meeting of the Orthopaoedic Research Society

After Fig. 1 and 2 in Sun, Y. et al. "Mechanical Properties of Single Type II Collagen Molecule." Paper No: 0082, 48th Annual Meeting of the Orthopaoedic Research Society. Image removed due to copyright considerations. Figure 1 in Fujii, T. et al. "Mechanical Properties of Single Hyaluronan Molecules." Paper No: 0390, 48th Annual Meeting of the Orthopaoedic Research Society

Modeling Extreme EXtension Ising Model Cizeau viovy 1997 Images removed due to copyright considerations

Modeling Extreme Extension: Ising Model Cizeau & Viovy 1997 Images removed due to copyright considerations

Image removed due to copyright considerations Figure 1 in Leger, J. F et al. "Reca binding to a single double-stranded DNA molecule A possible role of DNA conformational fluctuations Proc. Natl. Acad. Sci. USA-Biophysics 95 (Oct 1998)pp. 12295-12299

Image removed due to copyright considerations. Figure 1 in Leger, J. F. et al. "RecA binding to a single double-stranded DNA molecule: A possible role of DNA conformational fluctuations." Proc. Natl. Acad. Sci. USA - Biophysics 95 (Oct 1998) pp. 12295-12299

Images removed due to copyright considerations Bennink, Martin L et al. " Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers. "Nature Structural Biology 8(7) July 2001

Images removed due to copyright considerations. Bennink, Martin L. et al. "Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers." Nature Structural Biology 8 (7) July 2001

N EEEo>2 N SEE 三9=9三 日 是9

Pulling on Titin Sawtooth profile Image removed due to copyright considerations Figure 1 in Marszalek, Piotr E, et al. "Mechanical unfolding intermediates in titin modules Nature402,100-103(04Nov1999)

Pulling on Titin "Sawtooth profile" Image removed due to copyright considerations. Figure 1 in Marszalek, Piotr E., et al. "Mechanical unfolding intermediates in titin modules." Nature 402, 100-103 (04 Nov 1999)