上海交通大学：《医学微生物》课程教学资源（PPT课件讲稿）英文版 Laboratory Diagnosis

Laboratory Diagnosis

1 Laboratory Diagnosis

Category of Sample Blood, Urine, Stool, nasal washing, nasal swab, throat swab, saliva sputum, rectal swab, vesicle fluid scraping or swab), tissue brain biopsy, cerebrospinal fluid, et al

2 Category of Sample • Blood, Urine, Stool, nasal washing, nasal swab , throat swab, saliva , sputum, rectal swab, vesicle fluid( scraping or swab), tissue ,brain biopsy, cerebrospinal fluid, et al

Laboratory Diagnosis Microscopy Identification Virus isolation and identification Detection of viral proteins( antigens and enzymes )] Detection of viral genetic material Serologic procedures

3 Laboratory Diagnosis • Microscopy Identification • Virus isolation and identification • Detection of viral proteins( antigens and enzymes)] • Detection of viral genetic material • Serologic procedures

Microscopy Identification Light microscopy Fluorescent microscopy Electron microscopy

4 Microscopy Identification • Light microscopy • Fluorescent microscopy • Electron microscopy

Light microscopy Characteristic CPE Inclusion bodies

5 Light microscopy • Characteristic CPE • Inclusion Bodies

Cell death Cell rounding Degeneration Aggregation Loss of attachments to substrate Characteristic histological changes inclusion bodies in the nucleus or cytoplasm, margination of chromatin Syncytia: multinucleated giant cells caused by virus induced cell-cell fusion

6 • Cell death Cell rounding Degeneration Aggregation Loss of attachments to substrate • Characteristic histological changes:inclusion bodies in the nucleus or cytoplasm, margination of chromatin • Syncytia: multinucleated giant cells caused by virus￾induced cell-cell fusion

Fluorescent microscopy Fluorescent-antibody staining

7 Fluorescent microscopy • Fluorescent-antibody staining

Electron microscopy Direct detection: Human rotavirus: HAV HBV, Smallpox virus; Herpes virus Immune Electron micros copy Human rotavirus: HAV

8 Electron microscopy • Direct detection : Human rotavirus; HAV; HBV; Smallpox virus; Herpes virus. • Immune Electron microscopy: Human rotavirus; HAV;

Laboratory Diagnosis Microscopy Identification Virus isolation and identification Detection of viral proteins( antigens and enzymes )] Detection of viral genetic material Serologic procedures

9 Laboratory Diagnosis • Microscopy Identification • Virus isolation and identification • Detection of viral proteins( antigens and enzymes)] • Detection of viral genetic material • Serologic procedures

Viral isolation and dentification Viral growth and Cell culture Viral detection Viral Identification Interpretation of culture results

10 Viral isolation and Identification • Viral Growth and Cell culture • Viral Detection • Viral Identification • Interpretation of culture results