苏州大学医学部药学院：大类基础课程《生物化学实验 Biochemistry V Experiment》课程教学资源（电子教案）

苏州大学医学部 教 案 课程名称： Biochemistry V Experiment 授课对象： 2019级药学 授课学时： 36 Experimental Biochemistry Essentials 教材版本： 生物化学与分子生物学实验教程徐 岚、钱晖科学出版社，2014 主讲教师： 刘晶晶/高媛 苏州大学基础医学与生物科学学院制 2020年09月20日

苏州大学医学部 教 案 课程名称： Biochemistry V Experiment 授课对象： 2019 级药学 授课学时： 36 教材版本： Experimental Biochemistry Essentials 生物化学与分子生物学实验教程 徐 岚、钱晖 科学出版社，2014 主讲教师： 刘晶晶/高媛 苏州大学基础医学与生物科学学院制 2020 年 09 月 20 日

Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.10. 23/Fri/5-8 Chapter Text page:N/A Teaching duration:200 Title Introduction to basic techniques of biochemistry experiment 1.Understand the principle of centrifugation and know how to manipulate centrifuge Teaching goal 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3.Learn to use pipets 1、 Content: (1)入Lab safty training实10 (2)Lab report writing requirement 10 Teaching content、 (3)Use glass pipet 10 (4)Principle of centrifugation,types of centrifuge and how to use centrifuge,lab Teaching nethod、 practice I 30' (5),Principle of spectrophotometer,learn to use spectrophotometer,lab practice 2 Teaching emphasis、 30 difficulty、 (6)Student practices 110' time distribution 2.Key point:Learn to use centrifuge and spectrophotometer. Teaching method:Teacher should explain and display the experiment according to powerpoint Teaching Powerpoint,glass pipet,centrifuge,spectrophotometer instrument

Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 10. 23/Fri/5-8 Chapter 1 Text page：N/A Teaching duration: 200 Title Introduction to basic techniques of biochemistry experiment Teaching goal 1.Understand the principle of centrifugation and know how to manipulate centrifuge 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3. Learn to use pipets Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、 Content： （1）、Lab safty training 实 10‘ （2）、Lab report writing requirement 10‘ （3）Use glass pipet 10‘ （4）、Principle of centrifugation, types of centrifuge and how to use centrifuge，lab practice I 30’ （5）、Principle of spectrophotometer, learn to use spectrophotometer, lab practice 2 30’ （6）、Student practices 110’ 2、 Key point：Learn to use centrifuge and spectrophotometer。 Teaching method：Teacher should explain and display the experiment according to powerpoint . Teaching instrument Powerpoint,glass pipet, centrifuge, spectrophotometer

1. Will look down or look up the scale affect the volume during pipetting Question and quiz in 2.In room temperature,is the absorption proportional to the concentration of the class solution? After class 1.Emphasize the importance to balance the sample before centrifugation summary 2.Emphasize the use proper blank to calibrate spectrophotometer

Question and quiz in class 1. Will look down or look up the scale affect the volume during pipetting 2. In room temperature, is the absorption proportional to the concentration of the solution? After class summary 1. Emphasize the importance to balance the sample before centrifugation 2. Emphasize the use proper blank to calibrate spectrophotometer

Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.10 30/Fri/5-8 Chapter 3 Text page:N/A Teaching duration:200 Title Effects of some factors on enzymatic activity (a-salivary amylase) Teaching 1.Understand the properties of enzyme goal 2.Understand how factors such as temperature and pH affect enzymatic activity. l、Content: 1.Review mechanism of action of enzymes 10' 2.Review how temperature affect the Km and reaction speed 5' 3.Review how pH affect the Km of enzyme 5' Teaching 4. Review types of inhibitors,and how inhibitor affect the Km and Vmax of content、 enzyme 10 Teaching 5.How to design assays to test enzymatic activity.5' method、 6.Activity of amylase and assay to test amylase activity 5' Teaching 7.Experimental procedures 10' emphasis、 difficulty、 8.Students'experiment 150' time distribution 2、Key point: make sure saliva should be added the same time for all experimental groups 3.Teaching method:Teacher should explain and display the experiment according to powerpoint. Teaching Powerpoint,,glass pipet, instrument

Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 10. 30/Fri/5-8 Chapter 3 Text page：N/A Teaching duration: 200 Title Effects of some factors on enzymatic activity (a-salivary amylase) Teaching goal 1.Understand the properties of enzyme 2.Understand how factors such as temperature and pH affect enzymatic activity. Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、 Content： 1. Review mechanism of action of enzymes 10’ 2. Review how temperature affect the Km and reaction speed 5’ 3. Review how pH affect the Km of enzyme 5’ 4. Review types of inhibitors, and how inhibitor affect the Km and Vmax of enzyme 10’ 5. How to design assays to test enzymatic activity. 5’ 6. Activity of amylase and assay to test amylase activity 5’ 7. Experimental procedures 10’ 8. Students’ experiment 150’ 2、 Key point： make sure saliva should be added the same time for all experimental groups 3、Teaching method： Teacher should explain and display the experiment according to powerpoint . Teaching instrument Powerpoint,,glass pipet

Question 1.Name the types of inhibitors according to the mechanism of action? and quiz in class 2.How temperature/pH affect enzyme activity? After class When testing the effect of low temperature,make sure to put the tubes on ice summary immediately after adding saliva

Question and quiz in class 1. Name the types of inhibitors according to the mechanism of action? 2. How temperature/pH affect enzyme activity? After class summary When testing the effect of low temperature, make sure to put the tubes on ice immediately after adding saliva

Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.11. 06/Fri/5-8 Chapter Text page:N/A Teaching duration:200 Title The Lowry Assay for Protein quantification 1.Understand the principle of centrifugation and know how to manipulate centrifuge Teaching goal 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3.Learn to use pipets l、Content: (1),Principle of Lowry assay 20 (2),Procedure of protein measurement 30 Teaching content、 (3).Review how to use of spectrophotometer 10 Teaching nethod、 (4).Student practices 140 Teaching emphasis、 difficulty、 2、 Key point: (1).Principle of the Lowry assay time (2).Understand the linear range of the assay distribution (3).Copper reagent need to be prepared fresh.Upon adding reagent II need to be mixed immediately Teaching method:Teacher should explain and display the experiment according to powerpoint

Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 11. 06/Fri/5-8 Chapter 1 Text page：N/A Teaching duration: 200 Title The Lowry Assay for Protein quantification Teaching goal 1.Understand the principle of centrifugation and know how to manipulate centrifuge 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3. Learn to use pipets Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、 Content： （1）、Principle of Lowry assay 20‘ （2）、Procedure of protein measurement 30‘ （3）、Review how to use of spectrophotometer 10’ （4）、Student practices 140’ 2、 Key point： (1). Principle of the Lowry assay (2). Understand the linear range of the assay (3). Copper reagent need to be prepared fresh. Upon adding reagent II need to be mixed immediately Teaching method: Teacher should explain and display the experiment according to powerpoint

Teaching PPT,glass pipet,spectrophotometer instrument Question and quiz in 1.Other method to measure protein concentration? class After class Make sure that copper reagent should be prepared afresh. summary Make sure that all standards should be prepared at the same time

Teaching instrument PPT, glass pipet, spectrophotometer Question and quiz in class 1、Other method to measure protein concentration? After class summary Make sure that copper reagent should be prepared afresh. Make sure that all standards should be prepared at the same time

Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.11. 13/Fri/5-8 Chapter 4 Text page:N/A Teaching duration:200 Title Determination of Cholesterol In HDL (1).Master the technique to separate HDL from other types of lipoproteins Teaching goal (2).Understand the method to extract and determine cholesterol concentration in HDL (3).Learn the clinical significance of HDL l、Content: (1).Review the biochemistry of lipoprotein 20' Teaching (2).The method to separate HDL from other type of lipoprotein content、 10 Teaching (3).The principle of cholesterol extraction and quantification method、 20 Teaching (4)Student's experiment 150 emphasis、 difficulty、 2、Key point: (1).Significance of Lipoprotein. time distribution (2).Cholesterol extraction method 3.Teaching method Teacher should explain and display the experiment according to powerpoint Teaching Powerpoint,glass pipet,centrifuge,spectrophotometer instrument 1. What kinds of lipoprotein are found in our blood stream? Question and quiz in 2.What is the source and chemical content of these lipoproteins? class How to adjust diet for patients with increased blood cholesterol level

Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 11. 13/Fri/5-8 Chapter 4 Text page：N/A Teaching duration: 200 Title Determination of Cholesterol In HDL Teaching goal (1). Master the technique to separate HDL from other types of lipoproteins (2). Understand the method to extract and determine cholesterol concentration in HDL (3). Learn the clinical significance of HDL Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、Content： (1). Review the biochemistry of lipoprotein 20’ (2). The method to separate HDL from other type of lipoprotein 10’ (3). The principle of cholesterol extraction and quantification 20’ (4) Student’s experiment 150’ 2、Key point： (1). Significance of Lipoprotein. (2). Cholesterol extraction method 3、Teaching method ： Teacher should explain and display the experiment according to powerpoint . Teaching instrument Powerpoint,glass pipet, centrifuge, spectrophotometer Question and quiz in class 1. What kinds of lipoprotein are found in our blood stream? 2. What is the source and chemical content of these lipoproteins? 3. How to adjust diet for patients with increased blood cholesterol level

1.Solution should be mix well after adding phosphotungustate before adding magnesium chloride. After class 2.Sulfuric acid should be added drop by drop so thatthat all tubes are cooled summary the same. 3.Handle concentrated sulfuric acid with caution,in case of emergency, skin should be washed with running water for at least 15 minutes

After class summary 1. Solution should be mix well after adding phosphotungustate before adding magnesium chloride. 2. Sulfuric acid should be added drop by drop so that that all tubes are cooled the same. 3. Handle concentrated sulfuric acid with caution, in case of emergency, skin should be washed with running water for at least 15 minutes

Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.11. 20/Fri/5-8 Chapter 5 Text page:N/A Teaching duration:200 Title Purification of g-globulin From Serum(I) Teaching Understand the principle of chromatography Learn gel filtration techniques goal l、Content: (1),principle introduction 20' Chromatography(又名色谱)：A separation technique.The separation is based on differential partitioning between the mobile and stationary phases.Subtle Teaching differences in a compound's physic chemistry property result in differential content、 retention on the stationary phase and thus changing the separation Teaching method、 (2)、procedure100 1)Salt out Teaching 2)Packing emphasis、 difficulty、 3)Sample addition and Elution 4)Collection of the analytes time (3)Results and Discussion 40 distribution (4)、Reports40 2.Key point:principle for salt out protein from serum;principle for chromatography. 3 Teaching method:powerpoint and lab instrument. Teaching Powerpoint,glass pipet,centrifuge,spectrophotometer instrument

Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 11. 20/Fri/5-8 Chapter 5 Text page：N/A Teaching duration: 200 Title Purification of g-globulin From Serum (I) Teaching goal 1. Understand the principle of chromatography 2. Learn gel filtration techniques Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、 Content： （1）、principle introduction 20’ Chromatography(又名色谱):A separation technique. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's physic chemistry property result in differential retention on the stationary phase and thus changing the separation （2）、procedure 100‘ 1）Salt out 2）Packing 3）Sample addition and Elution 4）Collection of the analytes （3）Results and Discussion 40‘ （4）、Reports 40’ 2、 Key point ： principle for salt out protein from serum; principle for chromatography. 3、 Teaching method： powerpoint and lab instrument. Teaching instrument Powerpoint, glass pipet, centrifuge, spectrophotometer