武汉大学生命科学学院：《分子生物学》英文版 CHAPTER 9 The mutability and repair of DNA

Welcome each of You to My molecular Biology class @博来字 Wuhan University

Welcome Each of You to My Molecular Biology Class

Molecular Biology of the Gene 5/E --Watson et al. (2004) Part I: Chemistry and Genetics Part: Maintenance of the genome Part ll: Expression of the Genome Part IV: Regulation Part v: methods 3/22/05 Wuhan University

Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods 3/22/05

Part ll: Maintenance of the genome Dedicated to the structure of DNA and the processes that propagate,maintain and alter it from one cell generation to the next @博字 Wuhan University

Part II: Maintenance of the Genome Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next

Ch 6: The structures of dna and rna Ch 7: Chromosomes chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at he molecular level Ch 11: Site-specific recombination and transposition of DNA 3/22/05

Ch 6: The structures of DNA and RNA Ch 7: Chromosomes, chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA 3/22/05

Molecular iology Course CHAPTER 9: The mutability and repair of dna @肃博去字 yright( 2004 Pearson Education, Inc, publishing as Benjamin Cummings Wuhan Universit

CHAPTER 9: The mutability and repair of DNA •Molecular Biology Course

Different changes of DNA behavior re- address〔行为矫正) (death and unhealthy), Wnlchad Chapter8: Mutation(突变)isba needs to be repaired Chapter9: Recombination(重组) is good (diversity in a species beautiful),which is promoted Chapter10: Transposition(转座) is not bad because it is not repaired(benefit?)

Different changes of DNA -behavior re-address (行为矫正） ◼ Chapter 8: Mutation (突变) is bad (death and unhealthy), which needs to be repaired ◼ Chapter 9: Recombination (重组) is good (diversity in a species￾beautiful), which is promoted ◼ Chapter 10: Transposition (转座) is not bad, because it is not repaired. (benefit?)

The consequence of high rates of mutation ■ Mutation in germ line(生殖细胞) would destroy the species ■ Mutation in soma(体细胞) would destroy the individual Maintenance of the correctness of the DNA sequence is definitely crucial for living organisms. Keeping the error rate as low as 10-10 is so expensive

The consequence of high rates of mutation ◼ Mutation in germ line (生殖细胞) would destroy the species ◼ Mutation in soma (体细胞) would destroy the individual. Maintenance of the correctness of the DNA sequence is definitely crucial for living organisms. Keeping the error rate as low as 10-10 is so expensive

Be fair(公正) and serious (严肃) to science The points that i absolutely do not agree with Waston et al. 1. Mutation is not good, it could not be responsible for biodiversity. 2. Transposition is different from mutation because(1) producing mechanism is different;(2)no mechanism to correct it;(2) existing in nature in a well controlled manner(10-5)。(物种起源)

Be fair (公正) and serious (严肃) to science The points that I absolutely do not agree with Waston et al. 1. Mutation is not good, it could not be responsible for biodiversity. 2. Transposition is different from mutation because (1) producing mechanism is different; (2) no mechanism to correct it; (2) existing in nature in a well￾controlled manner (10-5). (物种起源)

Two important sources for mutation (unavoidable) a Inaccuracy in DNA replication (10-7 is not accurate enough) Errors(错误) a Chemical damage to the genetic material (environment) Lesions(损害伤害 arose from spontaneous damage) Damage(损害伤害 caused by chemica agents and radiation

Two important sources for mutation (unavoidable) ◼ Inaccuracy in DNA replication (10-7 is not accurate enough) Errors (错误) ◼ Chemical damage to the genetic material (environment) Lesions (损害,伤害arose from spontaneous damage) Damage (损害,伤害 caused by chemical agents and radiation

To repair an error or damage First, detect the errors Second, mend/repair the errors or lesions in a way to restore the original dNa sequence

To repair an error or damage First, Detect the errors Second, Mend/repair the errors or lesions in a way to restore the original DNA sequence