武汉大学生命科学学院：《分子生物学》英文版 CHAPTER 11 Site-Specific Recombination Transposition of DNA

Welcome each of you to My Molecular Biology class 博来字 Wuhan University

Welcome Each of You to My Molecular Biology Class

Molecular Biology of the Gene 5/E --Watson et al. (2004) Part I: Chemistry and Genetics Part: maintenance of the genome Part I: Expression of the Genome Part IV: Regulation Part v: methods

Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part ll: Maintenance of the genome edicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next Wuhan universit

Part II: Maintenance of the Genome Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next

Ch 6: The structures of dna and rna Ch 7: Chromosomes, chromatins and I the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA Wuhan University

Ch 6: The structures of DNA and RNA Ch 7: Chromosomes, chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA

Molecular iology Course Chapter 11 Site-Specific Recombination Transposition of DNA 博来字 Wuhan University

Chapter 11 Site-Specific Recombination & Transposition of DNA •Molecular Biology Course

There are genetic processes that rearrange DNA sequences and thus lead to a more dynamic genome structure

There are genetic processes that rearrange DNA sequences and thus lead to a more dynamic genome structure

Two classes of genetic recombination Conservative site-specific recombination (CSSR): recombination between two defined sequence elements Transpositional recombination (Transposition): recombination between specific sequences and nonspecific DNA sites

Two classes of genetic recombination • Conservative site-specific recombination (CSSR): recombination between two defined sequence elements • Transpositional recombination (Transposition): recombination between specific sequences and nonspecific DNA sites

Figure 11-1 site-specific recombination sites recombination transposable element transposition 十 Copyright 2004 Pearson Education, Inc. publishing as Benjamin Cummings

Figure 11-1

OUTLINE 1. Conservative Site-Specific Recombination 2. Biological Roles of Site-Specific Recombinatⅰon 3. Transposition 4. Examples of Transposable Elements and Their Regulation 5. VD)J Recombination

OUTLINE 1. Conservative Site-Specific Recombination 2. Biological Roles of Site-Specific Recombination 3. Transposition 4. Examples of Transposable Elements and Their Regulation 5. V(D)J Recombination

Topic 1: Conservative Site- Specific Recombination 1. Exchange of non-homologous sequences at specific DNa sites(what) 2. Mediated by proteins that recognize specific DNA sequences. (how)

Topic 1: Conservative Site￾Specific Recombination 1.Exchange of non-homologous sequences at specific DNA sites(what) 2.Mediated by proteins that recognize specific DNA sequences. (how)