北京大学：《细胞生物学 Cell Science》课程教学资源（PPT课件讲稿）第十二章 细胞社会学

细胞科 Cell science 12 蔡国平

细胞科学 Cell Science （12） 蔡国平

§12綱胞社会学- Cells into Tissues and organism 1.特殊的不同细胞群通过细胞黏附和细胞述接 形成多细胞( Multicellularity)组织 2.由组织构建器官 Extracellularmatrix,ECM)细胞外基质 不仅起着重要机械性作用,而且是重要组 织建成的组织者和和生理调节缓冲池 3.胞通祝和细胞信号传通使机体有了活 的灵魂

§ 12.细胞社会学- Cells into Tissues and organism 1.特殊的不同细胞群通过细胞黏附和细胞连接 形成多细胞（Multicellularity）组织 2.由组织构建器官- (ExtracellularMatrix, ECM) 细胞外基质 不仅起着重要机械性作用,而且是重要组 织建成的组织者和和生理调节缓冲池. 3.细胞通讯和细胞信号传递使机体有了活 的灵魂

S 12 Cell sociology: Cell association and interaction of between cells and their environment Multicellular organism: cellular and extracellular compartmwnt, cell surface is the boundary between two compartments Extracellular space extends outward from the outer surface of the plasma membrane and contains a variety of secreted materials that influence cell behaviour Extracellular. matrix: mainly, collagens proteoglycans, and a variety of non-collagen glycoproteins Extraneous structures: jelly coat, thick capsules around eggs, basement membrane, and the materials in which cartilage or bone cells reside

§ 12. Cell sociology: Cell association and interaction of between cells and their environment Multicellular organism: cellular and extracellular compartmwnt, cell surface is the boundary between two compartments. Extracellular space extends outward from the outer surface of the plasma membrane and contains a variety of secreted materials that influence cell behaviour. Extracellular matrix: mainly, collagens, proteoglycans, and a variety of non-collagen glycopreteins. Extraneous structures: jelly coat, thick capsules around eggs, basement membrane, and the materials in which cartilage or bone cells reside

12.1 Cell surface, a complex structural and functional system 1. Cell surface: Glycocalyx--cell coat and extracellular surface adherent materials Cytoplasmic membrane Gel layer underneath membrane Specialising structure of cell surface Cell junction

12.1 Cell surface, a complex structural and functional system 1. Cell surface: Glycocalyx—cell coat and extracellular surface adherent materials Cytoplasmic membrane Gel layer underneath membrane Specialising structure of cell surface Cell junction

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( Boundary and permeability barrier ② Organization and localization of function Nucleus 人 D Cell-to-cell communication (Transport processes ● Nutrients ④ Signal detection

av4 I 5a3 Iigure 430 The plasma membrane ef the human crythroote li Sanmin trophonsis(SDs- PAGE) used to fractionate the protrins of the erythmcyte sk of the gel. The ge has ben stained DorI 和出 Vard RckanlF Aka, a Hare wra cen B eL 4391. 1971: k ceney R加为都知k

Hypotonic medium xidase 一7 Figure 7-24 A Method For Labeling Proteins Exposed on One or oth Surfaces of a Membrane Vesicle. (a)When lactoperoxidase (LP) and 125) are present in the solution outside a membrane vesicle, LP catalyzes the labeling of membrane proteins exposed on the outer membrane surface (i.e. proteins A and B). If membrane vesicles are (b) first incubated in a hypotonic medium to make them permeable to LP and (c) then transferred to an isotonic solution containing 12I but no external LP proteins exposed on the inner membrane surface(i.e proteins B and C)become labeled

1) glycocalyx糖萼 (1)糖萼由细胞包被( cell coat- carbohydrate portions of membrane glycoproteins and glycolipids)和细胞外表面粘 附物质( extracellular surface adherent materials- proteoglycans, and a variety of glycoproteins)组成。前者 是指质膜糖蛋白或糖脂伸向胞外部分的糖链部分,虽然它们 是质膜的一个结构部分,但是它们在细胞外侧并且能够用糖 苷酶等将其移去而不使质膜或细胞受到致命性的损伤。后者 是处于包被外而又与包被紧密粘附的胞外基质(主要包括蛋 白多糖、糖蛋白等),实际上这两层结构界限不清,用钌红 染色时,染成一片,不易分辨开,因此细胞膜外缘的两层复 合物整体上称为糖萼( glycocalyx) (2)glycocalyx functions glycocalyx is thought to mediate cell-cell and cell- substratum interactions, provide mechanical protection to cells, and as a barrier to particles moving toward the plasma membrane

1) glycocalyx 糖萼 (1) 糖萼由细胞包被(cell coat – carbohydrate portions of membrane glycoproteins and glycolipids) 和细胞外表面粘 附 物 质 (extracellular surface adherent materials-- proteoglycans, and a variety of glycoproteins) 组成。前者 是指质膜糖蛋白或糖脂伸向胞外部分的糖链部分，虽然它们 是质膜的一个结构部分，但是它们在细胞外侧并且能够用糖 苷酶等将其移去而不使质膜或细胞受到致命性的损伤。后者 是处于包被外而又与包被紧密粘附的胞外基质（主要包括蛋 白多糖、糖蛋白等），实际上这两层结构界限不清，用钌红 染色时，染成一片，不易分辨开，因此细胞膜外缘的两层复 合物整体上称为糖萼(glycocalyx)。 (2) glycocalyx functions glycocalyx is thought to mediate cell-cell and cell￾substratum interactions,provide mechanical protection to cells, and as a barrier to particles moving toward the plasma membrane

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