麻省理工大学：《生物工程》教学讲义（英文版）lec 24 slid mig

Phases of cell gration□ Polarization□ Images removed due to copyright considerations Protrusion and adhesion Contraction[ Rear release l

Phases of Cell� Migration� • Polarization� • Protrusion and adhesion • Contraction� • Rear release� Images removed due to copyright considerations

Image removed due to copyright considerations Staining for actin (green)and myosin ii (red )in a migrating cell

Staining for actin (green) and myosin II (red) in a migrating cell. Image removed due to copyright considerations

Image removed due to copyright considerations Image removed due to copyright considerations Yellow indicates the location of myosin II tethered to the actin matrix in a migrating cell. o Actin polymerization generally occurs at the protruding membrane of a migrating cell

Yellow indicates the location of myosin II tethered to the actin matrix in a migrating cell.� Actin polymerization generally occurs at the protruding membrane of a migrating cell. Image removed due to copyright considerations. Image removed due to copyright considerations

Image removed due to copyright considerations See Figure 1 in Pollard, T D, and G.G. Borisy Cellular Motility Driven by assembly and Disassembly of Actin Filaments ce112:453465(21Feb2003

Image removed due to copyright considerations. See Figure 1 in Pollard, T. D., and G. G. Borisy. "Cellular Motility Driven by Assembly and Disassembly of Actin Filaments." Cell 112: 453-465 (21 Feb 2003)

One scenario for how myosin movement along the actin matrix can give rise to matriX contraction Dynamic network contraction actin filament b bipolar myosin II 2 2>>>>> c

One scenario for how myosin movement along the actin matrix can give rise to matrix contraction. bipolar myosin II actin filament

Cell migration patterns. In the absence of an external signal (e. g chemoattractant gradient). the mi gration pattern resembles a random walk Microvessel endothelial cells · Fibroblasts 9s900o 101 ⊥ mmm mm tm n Speed (um·miny) 100120140160180200220 X-position(μm) Migration speed tends to vary Inversely with persistence time

Cell migration patterns. In the absence of an external signal (e.g., chemoattractant gradient), the migration pattern resembles a random walk. Migration speed tends to vary inversely with persistence time

610F Mean detachment force(N Migration speed first increases, then falls as the strength of attachment is increased

Migration speed first increases, then falls as the strength of attachment is increased

Cilial Images removed due to copyright considerations Cilia beating on the surface of an airway epithelial cell

Cilia� Cilia beating on the surface of an airway epithelial cell. Images removed due to copyright considerations