《人体显微形态学》课程教学课件（PPT讲稿）The Structure And Usage of the Microscope

THE1StEXPERIMENTThe Structure And Usageof the Microscope

THE 1st EXPERIMENT The Structure And Usage of the Microscope

Readyour card carefully·Pleaseyourcarrymicroscopecarefully.> Hold the arm of the microscope with right hand, takethe base with the left hand, and put it in front of youbreastDon't take down any part of themicroscope

⚫Read your card carefully ⚫Please carry your microscope carefully. ➢ Hold the arm of the microscope with right hand, take the base with the left hand, and put it in front of your breast ⚫Don’t take down any part of the microscope

[Aims]To master the structure of themicroscope and learn how to use it. To know the cell's general shapeunder the microscope

[Aims] ⚫To master the structure of the microscope and learn how to use it. ⚫To know the cell’s general shape under the microscope

[Contents andManipulationsl. The Constitution ofThe Light Microscope

[Contents and Manipulations] 1. The Constitution of The Light Microscope

pointerOcular (eyepiece)Remagnifies theimageformedbythe objective lensOcularlensBody tube TransmitsLineof visiontheimagefromtheobjective lens to the ocularPathof lightPrismArmATC22BodytubeObjectivelensesPrimaryObjectivelensesswitchhundredfoldlenses thatmagnifythespecimenfortyfoldObjectiveStage Holds thepinchcocklensesdecuplemicroscope slideinpositionSpecimenCondenser FocusesknobCondenserlightthroughspecimenlensesDiaphragm ControlstheamountIlluminatorof lightenteringthecondenserCoarsefocusingknobBasewithlluminator Light sourcesourceafiluminatorBaseswitch(b)Thepathof light (bottomtotop)Fine focusingknob(a)Principalparts andfunctionsureA-6The Compound LightMicroscope.(a)Acompoundlightmicroscope.(b)Thepathoflightthroughthe compoundmicroscope

switch pointer hundredfold fortyfold decuple Objective lenses switch knob pinchcock

2. The Usage of The LightMicroscopeBefore using, we should check it iswhether complete and normal or notKeep 6-8cm to the edge of the desk

2. The Usage of The Light Microscope ⚫ Before using, we should check it is whether complete and normal or not. ⚫ Keep 6-8cm to the edge of the desk

(1)The Usageof The Low-Multiple ObjectiveLensAdjust the path of the light. Turn the objective lenses to make the low-multiple objective lens enter the path, and we could heard the sound of" ka"' at the same time.open the diaphragm, up the condenser,open twoeyes (adjust the distance between the two oculars), and use theilluminative control to make the light properPlace a slide in position on the stage and use the pinchcock to fix it.Pay attention to keep the side of the specimen up. Adjust the helix ofthe thruster to make the specimen go into the center of light path. Focus it. Turn the coarse focusing knob to make the stage up to thehighest, then turn the coarse focusing knob slowly to make the stagedown. When the image is in vision, turn the fine focusing knob tomake it in focus. Scan the slide with the thruster. Repeat abovemanipulations if the distance between the lens and the specimen ismore than 6.5mm

(1) The Usage of The Low-Multiple Objective Lens ⚫ Adjust the path of the light. Turn the objective lenses to make the low￾multiple objective lens enter the path, and we could heard the sound of “ ka” at the same time.open the diaphragm, up the condenser,open two eyes (adjust the distance between the two oculars), and use the illuminative control to make the light proper ⚫ Place a slide in position on the stage and use the pinchcock to fix it. Pay attention to keep the side of the specimen up. Adjust the helix of the thruster to make the specimen go into the center of light path. ⚫ Focus it. Turn the coarse focusing knob to make the stage up to the highest, then turn the coarse focusing knob slowly to make the stage down. When the image is in vision, turn the fine focusing knob to make it in focus. Scan the slide with the thruster. Repeat above manipulations if the distance between the lens and the specimen is more than 6.5mm

(2)TheUsageof The High-MultipleObjectiveLens Before use the high- multiple objective lens, youmust find the distinct image under low-multiple.move the target to the midpoint of the vision,and make it in focus. Turn the high- multiple objective lens enter the lightpath carefully,looking at the lens in order not todamage the specimen, then watch the specimen fromthe ocular by adjusting the fine focusing knob (Don'tuse the coarse focusing knob)

⚫ Before use the high- multiple objective lens, you must find the distinct image under low-multiple, move the target to the midpoint of the vision, and make it in focus. ⚫ Turn the high- multiple objective lens enter the light path carefully,looking at the lens in order not to damage the specimen, then watch the specimen from the ocular by adjusting the fine focusing knob (Don’t use the coarse focusing knob) . (2) The Usage of The High-Multiple Objective Lens

(3)TheUsageof The OilObjectiveLens Introduce it at the next class

⚫ Introduce it at the next class. (3) The Usage of The Oil Objective Lens

2. Observe the Cells' conformationHuman sperm slideBlood of toad slideSkeletal muscle slide smooth muscle slide

2. Observe the Cells’ conformation ⚫ Human sperm slide ⚫ Blood of toad slide ⚫ Skeletal muscle slide ⚫ smooth muscle slide