《人体显微形态学》课程教学课件（PPT讲稿）The Observation On The Organelle

THE 2ndEXPERIMENTThe Observation OnTheOrganelle

THE 2nd EXPERIMENT The Observation On The Organelle

[Aims]To master the usage of oil objective lens of themicroscope. To know the organelle's general shapes underthe microscope

[Aims] ⚫ To master the usage of oil objective lens of the microscope. ⚫ To know the organelle’s general shapes under the microscope

[Contentsand Manipulations1. the usage of the oil objective lensBefore use the oil lens, you must find the image under low power,1) move the target to the midpoint of the vision, and make it in focus.Move the low power lens(don't use the coarse focusing knob and2)the fine focusing knob ), drop the oil on the slide where you want towatch (one drop is enough)3)Turn the oil lens enter the path of the light carefully, and make thetip of the oil lens ioilimmerseinthecedar, while looking at the lens. Then watch the specimen from the ocularby turning the fine focusing knob (Don't use the coarse focusingknob)4)Clean the oil lens and the slide carefully with the special lens-paperby dipping the solution blending the aether and ethanol, then wipethe oil lens and the slide with dry lens paper again

[Contents and Manipulations] 1. the usage of the oil objective lens 1) Before use the oil lens, you must find the image under low power, move the target to the midpoint of the vision, and make it in focus. 2) Move the low power lens(don’t use the coarse focusing knob and the fine focusing knob ), drop the oil on the slide where you want to watch (one drop is enough) 3) Turn the oil lens enter the path of the light carefully, and make the tip of the oil lens immerse in the cedar oil , while looking at the lens. Then watch the specimen from the ocular by turning the fine focusing knob (Don’t use the coarse focusing knob) . 4) Clean the oil lens and the slide carefully with the special lens-paper by dipping the solution blending the aether and ethanol, then wipe the oil lens and the slide with dry lens paper again

2. Observe the organelles Golgi complex (the ganglion slide) Mitochondrion (the small intestine slide) centriole (the womb of bellyworm in the horse slide)

2. Observe the organelles ⚫ Golgi complex (the ganglion slide) ⚫ Mitochondrion (the small intestine slide) ⚫ centriole (the womb of bellyworm in the horse slide)

The arrows show Golgi complex of nerve cells inx100theganglion slide with brown granules

The arrows show Golgi complex of nerve cells in the ganglion slide with brown granules， × 100

The arrows show Golgi complex of nerve cells inthe ganglion slide with brown granules,× 1000

The arrows show Golgi complex of nerve cells in the ganglion slide with brown granules， × 1000

20051116163632Thearrows show Golgicomplex of nerve cells inthe ganglion slide with brown granules , × 400

The arrows show Golgi complex of nerve cells in the ganglion slide with brown granules， × 400

60352005the cross section slide of small intestine showmitochondrion with red granules 100 X

the cross section slide of small intestine show mitochondrion with red granules 100 ×

the cross section slide of small intestine showmitochondrion with red granules 100X

the cross section slide of small intestine show mitochondrion with red granules 100 ×

0503DHThe arrow show mitochondrion of the cross sectionslide for small intestine with red granules 400X

The arrow show mitochondrion of the cross section slide for small intestine with red granules 400 ×