《细胞分子生物学》（英文版）

S07100901 Molecular Biology of the Cell Part‖ Instructor:温龙平 lpwen@ustc.edu.cn

S07100901 Molecular Biology of the Cell Part II Instructor: 温龙平 lpwen@ustc.edu.cn

Topics I Cancer Stem Cells ■ Biomembranes ■ Nanobiology

Topics Cancer Stem Cells Biomembranes Nanobiology

Grading a Class attendance Discussion(20% a Team Presentation (40%) ■ Final exam(40%)

Grading Class Attendance & Discussion (20%) Team Presentation (40%) Final Exam (40%)

Presentations 5-member team(self-assembled) Select one paper(Science, Nature, Cell) within the fields of cancer, stem cell or apoptosis, discuss extensively by the team, and prepare for a 20-minute presentation(10-15 slides) Address the following in your presentation 1. Scientific question the paper was trying to answer 2. EXperimental approaches taken and main techniques used 3. Major findings and conclusions 4. Significance of the paper 5. Your ideas to further the research

Presentations 5-member team (self-assembled) Select one paper (Science, Nature, Cell) within the fields of cancer, stem cell or apoptosis, discuss extensively by the team, and prepare for a 20-minute presentation (10-15 slides) Address the following in your presentation: 1. Scientific question the paper was trying to answer; 2. Experimental approaches taken and main techniques used; 3. Major findings and conclusions; 4. Significance of the paper; 5. Your ideas to further the research

6000 Global cigarette consumption 5000 Tobacco 3000 32000 eases cue to wor and depresso 1000 Cancer 盛虽虽虽舀虽國虽 200 Global lung cancer deaths Caused by smoking 100 500 Non-tcbacco related 0 Nature Reviews I Cancer

Tobacco & Cancer

Cancer: The bottom line n animals, cell numbers are under exquisite control Loss of this control leads to proliferation of unwanted cells a Cancer arises when the above situation reaches a certain degree

Cancer: The Bottom Line In animals, cell numbers are under exquisite control Loss of this control leads to proliferation of unwanted cells Cancer arises when the above situation reaches a certain degree

Cancer vs tumor a Cancer cells are malignant tumor(vs benign tumor)cells a All cancer cells are eventually capable of metastasis

Cancer vs Tumor Cancer cells are malignant tumor (vs. benign tumor) cells All cancer cells are eventually capable of metastasis

Tissue Culture Systems ■ Primary Cell Cultures a Secondary Cell Strains Continuous cell lines

Tissue Culture Systems Primary Cell Cultures Secondary Cell Strains Continuous Cell Lines

Transformed cells ■ Reduced cell adhesion 1 Round-up morphology I Reduced serum requirements 1 Elevated hexose uptake 1 Anchorage-independent growth(ability to grow in semi-solid media, soft agar 1 Loss of density-dependent growth inhibition “ Contact Inhibition”) 1 Secretion of plasminogen activator 1 Capable of forming tumor in appropriete setting

Transformed Cells Reduced cell adhesion Round-up morphology Reduced serum requirements Elevated hexose uptake Anchorage-independent growth (ability to grow in semi-solid media,soft agar Loss of density-dependent growth inhibition (“Contact Inhibition”) Secretion of plasminogen activator Capable of forming tumor in appropriete setting

Causes of cancer Generalizations a Spontaneous genetic mutations Inherited cancer susceptibilities a Carcinogenic substances ■ Viruses ■ Radiation

Causes of Cancer Generalizations Spontaneous genetic mutations Inherited cancer susceptibilities Carcinogenic substances Viruses Radiation