同济大学：果蝇的核小体定位与基因调控（PPT讲稿，生命科学与技术学院：江赐忠）

果蝇的核小体定位与基因调控 江赐忠 同济大学生命科学与技术学院 The school of lile Science and technology LUNI 2021年2月1日

The School of Life Sciences and Technology Tongji University 果蝇的核小体定位与基因调控 江赐忠 同济大学生命科学与技术学院 2021年2月1日

What is nucleosome? Acetylation 2. Methylation 3. Ubiquination 4. Sumoylation 5. Phosphorylation 6. ADP ribosylation 7. Deimination 8. Proline isomerization 8-Histone Core H2A, H2B, H3 and H4

What is nucleosome? H2A , H2B , H3 and H4 1. Acetylation 2. Methylation 3. Ubiquination 4. Sumoylation 5. Phosphorylation 6. ADP ribosylation 7. Deimination 8. Proline isomerization

DNA packaging DNA Histone tails Histones Nucleosome Chromosome Modified from Nature 2008. 454: 711-715

DNA Nucleosome Chromosome Modified from Nature 2008, 454:711-715 DNA packaging

Nucleosome and gene regulation 久表及 TSS 从力 Pol ll ISS TBP-LTFIB

Nucleosome and gene regulation

One genome, multiple epigenomes 红细胞 平滑肌细胞 神经细胞 脂肪细胞 小肠表皮细胞

红细胞 神经细胞 脂肪细胞 小肠表皮细胞 平滑肌细胞 One genome, multiple epigenomes

Model organism Drosophila pupa embr yo larva egg adult

egg embryo larva pupa adult Model organism: Drosophila

MNase digest to remove linker DNA Harvest Isolate chromatin 事算 mbryo w/ H2A.Z antibodies EDSel Linke single molecule cap fure emPCR BLAST = 〓日2P晶即品暑 M ethods

Methods

Screen shot of browser 8334000833500083360008337000833800083390008340000834100083420008343000 12 10 86420 A n~n⊥几 5 WMM、AA 0 N8333822N8334945 ■N33766N838 342268N834 □H2A,ZNuc| exosomes CG7083-Ra DNApol-alpha50-RA CG7112-RB CG7182-RA Chromosome 3L

Screen shot of browser Chromosome 3L

Aggregation of nucleosome positions TSS

Nature 446 (7135):572-6 (2007) TSS Aggregation of nucleosome positions

Nucleosome organization around TSs gene 40 ∈35 30 25 三020 15 c 8-10 艺 -1000 500 0 500 1000 Nucleosome distance from Tss(bp)

Nucleosome organization around TSS gene +1 +2 +3 +4 +5 NFR