《Techniques of Molecular Biology》

Chapter 20 Techniques of Molecular Biology

Techniques of Molecular Biology Chapter 20

OUTLINE iNtroduction(p 647) Nucleic Acids(p. 648) Proteins(p, 672)

OUTLINE Introduction(p.647) Nucleic Acids(p.648) Proteins(p.672)

INTRODUCTION Understanding how the genetic processes of the cell work requires powerful, and complementary experimental approaches including the use of suitable model organisms in which the tools of genetic analysis are available

INTRODUCTION Understanding how the genetic processes of the cell work requires powerful,and complementary experimental approaches including the use of suitable model organisms in which the tools of genetic analysis are available

Methods for separating individual macromolecules from the myriad mixtures found in the celland for dissecting the genome specifiC DNA sequences

Methods for separating individual macromolecules from the myriad mixtures found in the cell,and for dissecting the genome specific DNA sequences

r Using computational or computational or bioinformatics approaches, to undertake large-scale genomic comparisons of both the casing and noncoding regions of various organIsms. r The methods of molecular biology depend upon and were developed from, an understanding of the properties of biological macromolecules themselves

Using computational or computational or bioinformatics approaches,to undertake large-scale genomic comparisons of both the cosing and noncoding regions of various organisms. The methods of molecular biology depend upon,and were developed from,an understanding of the properties of biological macromolecules themselves

A NOTEr It is important to appreciate that when we talk about isolating and purifying a given macromolecule in the ensuing discussion we rarely mean that a single molecule is isolated. rather the goal of these procedures is to isolate a large population of identical molecules from all of the other kinds of molecules in cell

A NOTE: It is important to appreciate that when we talk about isolating and purifying a given macromolecule in the ensuing discussion we rarely mean that a single molecule is isolated.rather,the goal of these procedures is to isolate a large population of identical molecules from all of the other kinds of molecules in cell

NUCLEIC ACIDS

NUCLEIC ACIDS

1.1 Electrophoresis through a Gel separates DNA and RNA Molecules According to Size This part is devoted to techniques for the manipulation and characterization of nucleic acids from the isolation of rnas and DNAs to the sequencing of entire genomes and comparative genomICs

This part is devoted to techniques for the manipulation and characterization of nucleic acids,from the isolation of RNAs and DNAs to the sequencing of entire genomes and comparative genomics. 1.1 Electrophoresis through a Gel separates DNA and RNA Molecules According to Size

Gel electrophoresis(凝胶电泳) separation of dna and rna molecules. Linear dna molecules separate according to size when subject to an electric field through a gel matrix(an inert jello-like porous material)动画

Gel electrophoresis (凝胶电泳) separation of DNA and RNA molecules.Linear DNA molecules separate according to size when subject to an electric field through a gel matrix(an inert,jello-like porous material)动画

DNA molecules are flexible and occupy an effective volume pores in the gel matrix sieve the dna molecules according to this volume; large molecules migrate more slowly through the gel because they have a larger effective volume than do smaller dNas and thus have more difficulty passing through the interstices of the gel Different sizes are separated because they moved different distances through the gel

DNA molecules are flexible and occupy an effective volume pores in the gel matrix sieve the DNA molecules according to this volume;large molecules migrate more slowly through the gel because they have a larger effective volume than do smaller DNAs,and thus have more difficulty passing through the interstices of the gel. Different sizes are separated because they moved different distances through the gel