麻省理工学院：《动物遗传学 Genetics》课程教学资源（英文讲义）Lecture 33_2

Oncogene dominant gain-of-function mutations promote cell transformation Tumor suppressor gene recessive,loss-of-function mutations promote cell transformation

Oncogene — dominant gain-of-function mutations promote cell transformation Tumor suppressor gene — recessive, loss-of-function mutations promote cell transformation

Images removed due to copyright reasons

Images removed due to copyright reasons

Daughter cells Extracellular growth control signals Go Intracellular quality control checks M(Mitosis) G1 G2 S (DNA synthesis) Figure by MIT OCW

growth checks (DNA synthesis) S G1 G0 G2 Extracellular control signals Intracellular quality control Daughter cells M (Mitosis) Figure by MIT OCW

Cell cycle in S.cerevisiae Mother cell Cytokinesis Daughter cell Chromosome segregation; Growth nuclear division M G1 START Spindle pole body duplication Spindle formation Bud emergence DNA replication Nuclear migration Figure by MIT OCW

Cell cycle in S. cerevisiae Mother cell Growth Spindle pole body duplication Spindle formation Chromosome segregation; Cytokinesis M G1 G2 S Daughter cell START Bud emergence DNA replication Nuclear migration nuclear division Figure by MIT OCW

Yeast in liquid culture Incubate at 23 C for 5 h Add mutagen; distribute into 0000000 smaller aliquots Plate out individual Colonies aliquots Agar Incubate at 23C Temperature-sensitive Replica-plate and for growth;growth at23°， incubate no growth at 36 23C 36℃ A Genetic Screen for Cell Cycle Mutants Figure by MIT OCW

A Genetic Screen for Cell Cycle Mutants Figure by MIT OCW