北京大学：《细胞生物学 Cell Science》课程教学资源（PPT课件讲稿）The cytoskeleton and cell mobility

细胞科学 el science 蔡国平

细胞科学 Cell Science （10.1） 蔡国平

The terminus of growing axon from the sea hare Aplysa, MF in blue, MT in red

The terminus of growing axon from the sea hare Aplysa, MF in blue, MT in red

S10 The cytoskeleton and cell mobility 10.1 Outline L. what is cytoskeleton An important characteristic of died eukaryote cell is Brownian motion of contents(including organelles) of these cells, while the organelles and subcellular particles are orientally organised and carry out oriental motion and order metabolitic activities. And a variety of cells carry out different functions with special type of movement and cell shape or conformation. Raising these characters and performing these processes are mediated by cytoskeleton

§10 The cytoskeleton and cell mobility 10.1 Outline 1.what is cytoskeleton An important characteristic of died eukaryote cell is Brownian motion of contents (including organelles) of these cells, while the organelles and subcellular particles are orientally organised and carry out oriental motion and order metabolitic activities. And a variety of cells carry out different functions with special type of movement and cell shape or conformation. Raising these characters and performing these processes are mediated by cytoskeleton

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IF(vimentin) MT ME

IF(vimentin) MT MF

tubulin vimentin fluorescein coumarin rhodamine Figure 9.6 Fluorescence localization of microfilaments, antibodies. The fluorescent conjugate used in each case is microtubules, and intermediate filaments in the same cell. indicated in the lower right of each photo. Each of these l

(a)Microtubules (b)Microfilaments Figure 22-1 The Intracellular Distribution of Microtubules Microfilaments, and Intermediate Filaments. (a) The distribution of microtubules in cells of the kangaroo rat kidney cell line(PtK-1l visualized by immunofluorescence staining for tubulin For reference, the ucleus has been stained with the red fluorescent DNA stain propidi odide. (b) The distribution of microfilaments in a rat kidney cell line visualized by staining actin with a fluorescent derivative of phalloidin (c) The distribution of intermediate filaments in PtK-1 cells, visualized by immunofluorescence staining for keratin. Here the nucleus has also been (c)Intermediate filaments 5um stained with propidium iodide

The cytoskeleton is composed of three well defined filamentous structures- microtubles: 25 nm in diameter; microfilaments, 7 to 9 nm in diameter; and intermediate filaments. 10 nm in diameter-that together form an elaborate interactive network, which is also associated with the nuclear matrix and cytoplasm membrane. They are a highly dynamic group of structures capable of rapid and dramatic reorganization. In addition, many of functions of the cytoskeleton require a host of accessory proteins, which may are not a part of the filaments themselves

The cytoskeleton is composed of three well￾defined filamentous structures- microtubles, 25 nm in diameter; microfilaments, 7 to 9 nm in diameter; and intermediate filaments, 10 nm in diameter-that together form an elaborate interactive network, which is also associated with the nuclear matrix and cytoplasm membrane. They are a highly dynamic group of structures capable of rapid and dramatic reorganization. In addition, many of functions of the cytoskeleton require a host of accessory proteins, which may are not a part of the filaments themselves

About cytoplasmic matrix and microtrabeculae, the former is cytosolic gel-like phase, the later is a performance concept. Under high-voltage electron microscopy, in the cells untreated by detergent the principal cytoskeleton filaments appear to be extensively interconnected by a three-dimensional network of fine thread-like proteins that are removed in detergent-treated cells, this network is called microtrabeculae. However. so far its existence still is doubted, if it is caused by aggregation of cytosolic proteins in the course of performing cells

About cytoplasmic matrix and microtrabeculae, the former is cytosolic gel-like phase, the later is a performance concept. Under high-voltage electron microscopy, in the cells untreated by detergent the principal cytoskeleton filaments appear to be extensively interconnectrd by a three-dimensional network of fine thread-like proteins that are removed in detergent-treated cells, this network is called microtrabeculae. However, so far its existence still is doubted, if it is caused by aggregation of cytosolic proteins in the course of performing cells

Deep-etch EM in flc treated with nonionic dete gative stain EM glial cell treated with nonionic detergent High-voltage EM of flc treated Deep-etch EM in with detergent rat axon treate with deter High-voltage eM of microtrabecular network

Deep -etch EM in FLC treated with nonionic detergent Negative stain EM in glial cell treated with nonionic detergent High -voltage EM of microtrabecular network High -voltage EM of FLC treated with detergent Deep -etch EM in rat axon treated with detergent