清华大学：《分子生物学》（英文版）Chapter 12 The replicon

Chapter 12 The replicon 莘大

Chapter 12 The replicon

12.1 Introduction 12.2 Replicons can be linear or circular 12.3 Origins can be mapped by autoradiography and electrophoresis 12. 4 The bacterial genome is a single circular replicon 12.5 Each eukaryotic chromosome contains many replicons 12.6 Isolating the origins of yeast replicons 12.7 D loops maintain mitochondrial origins 2.8 The problem of linear replicons 12.9 Rolling circles produce multimers of a replicon 12. 10 Rolling circles are used to replicate phage genomes 12. 11 The f plasmid is transferred by conjugation between bacteria 2. 12 Conjugation transfers single-stranded dNA 12. 13 Connecting bacterial replication to the cell cycle 12 14 Cell division and chromosome segregation 2. 15 The division apparatus consists of cytoskeletal and regulatory components 2. 16 Partitioning involves membrane attachment and(possibly )a motor 12. 17 Multiple systems ensure plasmid survival in bacterial populations 12. 18 Plasmid incompatibility is determined by the replicon 12 19 The ColE l compatibility system is controlled by an RNA regulator 消当

12.1 Introduction 12.2 Replicons can be linear or circular 12.3 Origins can be mapped by autoradiography and electrophoresis 12.4 The bacterial genome is a single circular replicon 12.5 Each eukaryotic chromosome contains many replicons 12.6 Isolating the origins of yeast replicons 12.7 D loops maintain mitochondrial origins 12.8 The problem of linear replicons 12.9 Rolling circles produce multimers of a replicon 12.10 Rolling circles are used to replicate phage genomes 12.11 The F plasmid is transferred by conjugation between bacteria 12.12 Conjugation transfers single-stranded DNA 12.13 Connecting bacterial replication to the cell cycle 12.14 Cell division and chromosome segregation 12.15 The division apparatus consists of cytoskeletal and regulatory components 12.16 Partitioning involves membrane attachment and (possibly) a motor 12.17 Multiple systems ensure plasmid survival in bacterial populations 12.18 Plasmid incompatibility is determined by the replicon 12.19 The ColE1 compatibility system is controlled by an RNA regulator

12.1 Introduction Replicon is a unit of the genome in which DNA is replicated; contains an origin for initiation of replication 消当

Replicon is a unit of the genome in which DNA is replicated; contains an origin for initiation of replication. 12.1 Introduction

12.1 Introduction Replicon is a unit of the genome in which DNA is replicated; contains an origin for initiation of replication 消当

Replicon is a unit of the genome in which DNA is replicated; contains an origin for initiation of replication. 12.1 Introduction

12.1 Introduction Type of Unit Genome structure Mode of Propagation Cneq山 ences Lytic phage ds- or ss-DN A or RNA Infects susceptible host Usually kills host li near or circular Lysogenic phage ds-DNA Linear sequence in Im munity to infection host chromo some Plasmid ds-DNA cirde Replicate s at defined Im munity to plasmids May be transmissible In same group Episome ds-DNA circle Free circle or linear integrated May transfer host DNA Figure 11.2 Several types of independent genetic units exist in bacteria 消当

Figure 11.2 Several types of independent genetic units exist in bacteria. 12.1 Introduction

12.2 Origins can be mapped by autoradiography and electrophoresis Replication fork is the point at which strands of parental duplex dna are separated so that replication can proceed 消当

Replication fork is the point at which strands of parental duplex DNA are separated so that replication can proceed. 12.2 Origins can be mapped by autoradiography and electrophoresis

Nonreplicated Replication eye Nonreplicated 12.2 Origins can be dNA DNA mapped by Appearance autoradiography and electrophoresis Figure 12.1 Replicated DNA is seen as Molecular structure a replication eye fl d bv anked by nonreplicated DNA 消当

Figure 12.1 Replicated DNA is seen as a replication eye flanked by nonreplicated DNA. 12.2 Origins can be mapped by autoradiography and electrophoresis

UNIDIRECTIONAL REPUCATION 12.2 Origins can be mapped by autoradiography Replication fork and N electrophoresis 八AMmA Parental DNA\ Figure 12.2 Replicons BIDIRECTIONAL REPLICATION may be unidirectional or bidirectional depending on whether one or two replication Replication fork Replication fork forks are formed at the origin A 入 请莘大

Figure 12.2 Replicons may be unidirectional or bidirectional, depending on whether one or two replication forks are formed at the origin. 12.2 Origins can be mapped by autoradiography and electrophoresis

12.2 Origins can be mapped by autoradiography and electrophoresis Replicating e structure Appearance of e structure by electron miscopy Figure 12. 3 a replication eye forms a theta structure in circular dna 消当

Figure 12.3 A replication eye forms a theta structure in circular DNA. 12.2 Origins can be mapped by autoradiography and electrophoresis

12.2 Origins can be mapped by autoradiography and electrophoresis Figure 12. 4 The replication eye becomes larger as the replication forks proceed along the replicon. Note that the eye becomes larger than the nonreplicated segment. The two sides of the eye can be defined because they are both the same length. Photograph kindly provided by bernard Hirt 消当

Figure 12.4 The replication eye becomes larger as the replication forks proceed along the replicon. Note that the "eye" becomes larger than the nonreplicated segment. The two sides of the eye can be defined because they are both the same length. Photograph kindly provided by Bernard Hirt. 12.2 Origins can be mapped by autoradiography and electrophoresis