河北医科大学：《天然药物化学》课程教学资源（课件讲稿）Introduction of NMR、MS、X-ray授课课件 3/5

2018/10/27 Saturday NMR Sensitivity To Increase Magnet Strength is a Major Means 11'10" 11 8'6 92 511 5.000.000 600 700 2001年 1995年 Applications: 核曦共振已在众多领域中有了广泛的应用.从技术手段上讲， 核磁共振的应用主要有两个方面： I核殖共振波谱：实际上是吸收率（纵坐标）对化学位移 (横坐标)的关系曲线 Ⅱ核磁共振成象RI):(I)点成像法 (2)弛豫时间成像法 Nuclear Magnetic Resonance Imaging also called Spin Imaging 122 1

2018/10/27 Saturday 1 NMR Sensitivity 2018/10/27 Saturday To Increase Magnet Strength is a Major Means 2001年 1995年 Applications: Ⅱ核磁共振成象(MRI)：(1) 点成像法 核磁共振已在众多领域中有了广泛的应用.从技术手段上讲， 核磁共振的应用主要有两个方面： Ⅰ核磁共振波谱：实际上是吸收率（纵坐标）对化学位移 （横坐标）的关系曲线 (2) 弛豫时间成像法 Nuclear Magnetic Resonance Imaging also called Spin Imaging 122

2018/10/27 Saturday Application of NMR Drug design Metabonomics Food quality Varian 600 MHz NMR Spectrometer 2

2018/10/27 Saturday 2 Application of NMR 123 Varian 600 MHz NMR Spectrometer 124

2018/10/27 Saturday Liquid Helium-cooled Magnet Bruker 700 MHz Avance III Spectrometer with carbon-optimized cryoprobe 3

2018/10/27 Saturday 3 Liquid Helium-cooled Magnet 125 Bruker 700 MHz Avance III Spectrometer with carbon-optimized cryoprobe 126 700 MHz

2018/10/27 Saturday 950U5 00 MHz 127 1000&1020MHz超导核磁共振波谱仪 020

2018/10/27 Saturday 4 127 900 MHz 128 1000 &1020 MHz超导核磁共振波谱仪

2018/10/27 Saturday co," 129 130 5

2018/10/27 Saturday 5 129 130

2018/10/27 Saturday Superconducting Magnet Insulation隔热材料 Liquid helium Liguid nitroge Sample 接买 Magnet legs 6

2018/10/27 Saturday 6 131 Superconducting Magnet Dewar 杜瓦瓶 Insulation 隔热材料 Liquid nitrogen Liquid helium Main magnet coil 主磁铁线圈 Magnet legs NMR detection probe 核磁共振探测探头 Sample

2018/10/27 Saturday Schematic operation of a basic NMR spectrometer and 射频振荡器 aaana8 射频接收器 控制台 ee 记录仪 磁体 apne3 磁体 样品管 Svesp enerator 扫描发生器 NMR组成 134 7

2018/10/27 Saturday 7 Schematic operation of a basic NMR spectrometer 133 磁 体 磁 体 样品管 射频振荡器 射频接收器 扫描发生器 记录仪 控制台 NMR组成 134

2018/10/27 Saturday NMR is A Widely Used Structural Tool Liquid-state NMR HO-CH,CH olid-state NM NMR spectrometer Superconducting magnet control NMR p 8

2018/10/27 Saturday 8 135 NMR is A Widely Used Structural Tool NMR spectrometer Superconducting magnet NMR console Rf generation and amplification Workstation Spectrometer control NMR probe

2018/10/27 Saturday 5mm(600L, 1.7 mm (35 pL)and I mm tubes (7 uL). Right,a gas-tight sample syringe fitted with an extra long needle Image credit:D.S.Dalisav Fig.1 Relative NMR sample tube diameter (OD)and sample volumes. From left to right. HNMR Integrate,Enlarge and Print 化学位移、裂分情况、锅合常教？ 9

2018/10/27 Saturday 9 5 mm (600 µL), 1.7 mm (35 µL) and 1 mm tubes (7 µL). Right, a gas-tight sample syringe fitted with an extra long needle. Image credit: D. S. Dalisay. Fig. 1 Relative NMR sample tube diameter (OD) and sample volumes. From left to right, Tadeusz F. Molinski. NMR of natural products at the ‘nanomole-scale’. Nat. Prod. Rep., 2010, 27, 321-329. 137 Integrate, Enlarge and Print 1H NMR 138 化学位移、裂分情况、耦合常数？

2018/10/27 Saturday NMR Spectral Parameters 装iag → Chemical shift 作用action J-coupling Relaxation Line-width 黑柏action → Nuclear Overhauser effect nOe 条g若oar couing RDC 屏蔽效应与去屏蔽效应 顺磁性位移，去屏蔽效应 抗磁性位移，屏蔽效应 T 高场 CHC13 8小 140 10

2018/10/27 Saturday 10 NMR Spectral Parameters nOe RDC Line-width Shielding 屏蔽 Chemical shift Scalar interaction 标量相互作用 J-coupling Nuclear Overhauser effect Dipolar interaction 偶极相互作用 Residual dipolar coupling 残余偶极偶合 Relaxation 屏蔽效应与去屏蔽效应 屏蔽作用（屏蔽效应）（shielding effect） 去屏蔽作用（去屏蔽效应）（deshielding effect） 140