《分子生物学》课程PPT教学课件（双语版）基因转录、调控、表达

Compact chromatin RNA polymerase H (H3H4)2H2AH2B hexamer H3 er H4 H4 H2B H3 H2A RNA polymerase

Form II B FormⅢ C

parental strand TTGGGGTTGGGGTTGGGGTT AACCCC 5 incomplete, newly synthesized lagging strand TELOMERASE BINDS TTGGGGTTGGGGTTGGGGTTG telomerase AACCCC CCCCAACI TELOMERASE EXTENDS 3 END (RNA-templated telomerase with bound RNA template DNA synthesis) TTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTT AACCCC CCCCAAC COMPLETION OF LAGGING STRAND BY DNA POLYMERASE DNA-templated DNA synthesis) TTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTG AACCCC CCCCAACCCCAACCCC DNA polymerase

Template strand Coding strand ATGCGCCATGCCAGTTACGTACATGGA TACGCGGTACGGTCAATGCATCTACCT RNA sequence is TRANSCRIPTION complementary to template strand identical to coding strand UACGCGGUACGGUCAAUGCAUCUACCU RNA transcript

nation Only one reading frame is open Termination Third reading frame is blocked Termination

NUCLEUS Exon 1 Exon 2 DNAAMNNAA Transcription mRNA S 9 RNA RIbosome RNA CYTOPLASM

MAMAVA DNA Leader Traile 5 -3 RNA N 00ooeoc Protein rotein defines coding reg Length of RNA defines region of gene

时e12,10 An ARS extends 5o bp and includes a consensus sequence and additiona element(B1-B3). 100 Mutations in B elements reduce origin function Mutations in core consensu sh ongin function

Nuclear pore complex 120nm Radial arms Coaxial rings Pore 10 nm Transporter Spokes Channel? 150nm Nuclear pore complex with radial arms