《病原生物学（人体寄生虫学）》课程实验课件（英文）01 protozo

Human ParasitologyDepartment of Pathogenic and ImmunologySHIHEZIUNIVERSITY

Department of Pathogenic and Immunology Department of Pathogenic and Immunology SHIHEZI SHIHEZI UNIVERSITY Human Parasitology

EntamoebaSchematic diagram of a protozoan cellTrophozoiteNuclear membranekaryosomeendoplasmen0ectoplasmecVesicular nuclearCell membraneECChromatin beadsAchromatic fibrilsDepartmentofPathogenicand Immunology

Schematic diagram of a protozoan cell Vesicular nuclear endoplasm ectoplasm Cell membrane Nuclear membrane Chromatin beads karyosome Achromatic fibrils Trophozoite Entamoeba Department of Pathogenic and Immunology Department of Pathogenic and Immunology

Trophozoite of Entamoeba histolyticnucleolusHighly activenucleus核膜Invading the核周染色质Inhabit inmucosa andthe lumen ofother viscerathe intestineRBC in foodvesicle核EntamoebaEncystations大滋养体dispardo not occur小滋养体

Trophozoite of Entamoeba histolytic nucleolus Highly active Highly active nucleus Invading the Invading the mucosa and mucosa and other viscera other viscera RBC in food RBC in food vesicle vesicle Encystations Encystations do not occur do not occur Inhabit in Inhabit in the lumen of the lumen of the intestine the intestine Entamoeba dispar

EntamoebaTrophozoite( with hematoxylin : (1) Size: 10-40 micrometers in diameter, some areabove 60 micrometers.: (2) Ectoplasm:transparentEndoplasm: granules. RBC may be found: (3) Nucleus (vesicular type)A: membrane: distinct lineB: chromatin granules: fine and uniformlyarranged in the inner surface of thenuclear membraneC: karyosome: small and centrally located.Department of Pathogenic and Immunology

Trophozoite ( with hematoxylin ) • (1) Size: 10 (1) Size: 10-40 micrometers in diameter, some are 40 micrometers in diameter, some are above 60 micrometers. above 60 micrometers. • (2) Ectoplasm:transparent (2) Ectoplasm:transparent Endoplasm: granules. RBC may be found. Endoplasm: granules. RBC may be found. • (3) Nucleus (vesicular type) (3) Nucleus (vesicular type) A: membrane: distinct line A: membrane: distinct line B: chromatin granules: fine and uniformly B: chromatin granules: fine and uniformly arranged in the inner surface of the arranged in the inner surface of the nuclear membrane. nuclear membrane. C: karyosome karyosome: small and centrally located. : small and centrally located. Entamoeba Department of Pathogenic and Immunology Department of Pathogenic and Immunology

(4) characteristic:· Pseudopodium : motility is progressive anddirectionalbroad or finger-like in formfirstly, formed with ectoplasmsecondly, endoplasm flows slowly into it.? lick up RBCDepartmentofPathogenicand Immunology

(4) characteristic: (4) characteristic: •Pseudopodium : motility is progressive and Pseudopodium : motility is progressive and directional directional broad or finger broad or finger-like in form firstly, formed with ectoplasm, firstly, formed with ectoplasm, secondly, endoplasm flows slowly into it. secondly, endoplasm flows slowly into it. • lick up RBC Department of Pathogenic and Immunology Department of Pathogenic and Immunology

karyosomeTrophozoiteNucleax membrane核周染色质粒Achromatic fibrilsChromatin beads核纤维endoplasm内质lick up RBCectoplasm外质DepartmentofPathogenicand Immunology

Trophozoite karyosome Nuclear membrane Chromatin beads endoplasm ectoplasm lick up RBC Achromatic fibrils Department of Pathogenic and Immunology Department of Pathogenic and Immunology

endosomenucleusDepartmentofPathogenicand Immunology

Department of Pathogenic and Immunology Department of Pathogenic and Immunology

Trophozoite

Trophozoite

Entamoeba histolyticasiteTrophozoitetronh070ute

Trophozoite

Entamoeba histolyticatrophozoite[by P.W. Pappas and S.M. WardropDepartmentofPathogenicand Immunology

Department of Pathogenic and Immunology Department of Pathogenic and Immunology