麻省理工大学：遗传学（Genetics）讲稿_lecture33_2

Oncogene一 dominant gain-of-function mutations promote cell transformation umor suppressor gene recessive, loss-of-function mutations promote cell transformation

Oncogene — dominant gain-of-function mutations promote cell transformation Tumor suppressor gene — recessive, loss-of-function mutations promote cell transformation

Images removed due to copyright reasons

Images removed due to copyright reasons

Daughter ce Extracellular rowth control signals Intracellula uality contro checks G (DNA synthesis) Figure by MIT OCW

growth checks (DNA synthesis) S G1 G0 G2 Extracellular control signals Intracellular quality control Daughter cells M (Mitosis) Figure by MIT OCW

Cell cycle in S cerevisiae Mother cell Cytokinesis Daughter cell Chromosome segregation Growth nuclear division START Spindle pole body duplication Spindle formation Bud emergence DNA replication Nuclear migration igure by MIT OCW

Cell cycle in S. cerevisiae Mother cell Growth Spindle pole body duplication Spindle formation Chromosome segregation; Cytokinesis M G1 G2 S Daughter cell START Bud emergence DNA replication Nuclear migration nuclear division Figure by MIT OCW

Yeast in liquid culture Incubate at 23 C for 5 h Add mutagen distribute into smaller aliquots Plate out dividual Colonies aliquots Agar Incubate at23°C Temperature-sensitive Replica-plate and for growth; growth at 23 incubate no growth at36° 23°C 36°C A Genetic Screen for Cell cycle Mutants Figure by MIT OCW

A Genetic Screen for Cell Cycle Mutants Figure by MIT OCW